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TRA-1-81 Antibody (MA1-024) in ICC/IF
Immunofluorescent analysis of TRA-1-81 using anti-TRA-1-81 monoclonal antibody (Product # MA1-024) shows staining on the cell surface of human H9 human embryonic stem cells, indicating pluripotency. TRA-1-81 staining (green) and an overlay image of TRA-1-81 with DAPI (blue) is shown. H9 human embryonic stem cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a Monoclonal antibody recognizing TRA-1-81 (Product # MA1-024) at a dilution of 1:20 overnight at 4 °C, washed with... View More
Product Details
MA1-024
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Bovine,
Human
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
TRA-1-81
Immunogen
human embryonal carcinoma cell line 2102Ep
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography - MBP
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536706
Target Information
TRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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