Invitrogen

TRAF3 Recombinant Rabbit Monoclonal Antibody (12H13L59)

TRAF3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

TRAF3 Antibody (700121) in ICC/IF

Immunofluorescent analysis of TRAF3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with TRAF3 Recombinant Rabbit Monoclonal Antibody (Product # 700121) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Pa... View More

TRAF3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

TRAF3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A549 cells were transfected with TRAF3 siRNA and loss of signal was observed in Western Blot using Anti-TRAF3 Monoclonal Antibody (Product # 700121). {KD}

Product Details

700121

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

12H13L59

Immunogen

A recombinant protein corresponding to amino acids 1-187 of Q13114.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2532283

Product Specific Information

This antibody is predicted to react with chimpanzee, Rhesus monkey, mouse, rat, porcine, canine, bovine and equine based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e. g. RANK, CD30, CD40, etc. ) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6). TRAF3, originally named CRAF1, interacts directly with the CD40 cytoplasmic tail through a region of similarity to the tumor necrosis factor-alpha (TNF-alpha) receptor-associated factors. TRAF3 binds only a single site, which contains the sequence PEQET, whereas TRAF1 and TRAF2 are capable of binding to either the PEQET site or an additional downstream domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A20 undermines alternative NF-κB activity and expression of anti-apoptotic genes in Helicobacter pylori infection.

Cellular and molecular life sciences : CMLS

Lim MCC,Maubach G,Birkl-Toeglhofer AM,Haybaeck J,Vieth M,Naumann M

700121 was used in Western Blot to report that the classical NF-κB target gene product A20 contributes to the negative regulation of alternative NF-κB signaling in gastric epithelial cells infected by Helicobacter pylori.

Fri Jan 28 00:00:00 UTC 2022

TIFA has dual functions in Helicobacter pylori-induced classical and alternative NF-κB pathways.

EMBO reports

Maubach G,Lim MCC,Sokolova O,Backert S,Meyer TF,Naumann M

Published figure using TRAF3 recombinant monoclonal antibody (Product # 700121) in Western Blot

Mon Sep 06 00:00:00 UTC 2021

Cellular inhibitors of apoptosis are global regulators of NF-κB and MAPK activation by members of the TNF family of receptors.

Science signaling

Varfolomeev E,Goncharov T,Maecker H,Zobel K,Kömüves LG,Deshayes K,Vucic D

Tue Mar 20 00:00:00 UTC 2012

Cellular inhibitors of apoptosis are global regulators of NF-κB and MAPK activation by members of the TNF family of receptors.

Science signaling

Varfolomeev E,Goncharov T,Maecker H,Zobel K,Kömüves LG,Deshayes K,Vucic D

Tue Mar 20 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: CD40 associated protein 1; CD40 binding protein; CD40 receptor associated factor 1; CD40 receptor-associated factor 1; CD40-binding protein; CD40BP; CRAF1; LAP1; LMP1-associated protein 1; RING-type E3 ubiquitin transferase TRAF3; TNF receptor-associated factor 3

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Gene Aliases: CAP-1; CAP1; CD40bp; CRAF1; IIAE5; LAP1; TRAF3; TRAFAMN

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UniProt ID: (Human) Q13114

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Entrez Gene ID: (Human) 7187

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Function(s)

ubiquitin-protein transferase activity signal transducer activity tumor necrosis factor receptor binding protein binding zinc ion binding ligase activity protein kinase binding protein phosphatase binding ubiquitin protein ligase binding thioesterase binding

Process(es)

regulation of cytokine production toll-like receptor signaling pathway apoptotic process signal transduction Toll signaling pathway protein ubiquitination regulation of proteolysis negative regulation of NF-kappaB transcription factor activity negative regulation of type I interferon production regulation of interferon-beta production tumor necrosis factor-mediated signaling pathway TRIF-dependent toll-like receptor signaling pathway regulation of apoptotic process innate immune response regulation of defense response to virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TRAF3 Recombinant Rabbit Monoclonal Antibody (12H13L59)

TRAF3 Antibody (700121) in ICC/IF

Product Details

700121

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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