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Invitrogen
TRAP1 Monoclonal Antibody (3H4-2H6), PerCP
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FIGURE: 1 / 2
TRAP1 Antibody (MA5-45310) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using Myoblast cell line C2C12. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Trap1 monoclonal antibody (Product # MA5-45310) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was DAPI (blue) nuclear stain at 1:5,000 for 5 min RT. Localization: Nucleus. Magnification: 60X.
Product Details
MA5-45310
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
3H4-2H6
Immunogen
Purified recombinant TRAP1
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931764
Product Specific Information
1 µg/mL of MA5-45310 was sufficient for detection of Trap-1/HSP75 in 20 µg of Human A431 lysate by ECL immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 75kDa.
Target Information
The 90 kDa heat shock protein (hsp90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. Hsp90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of hsp90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like hsp90, TRAP1 is found to be associated with numerous proteins involved in diverse actions. Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting presequence strongly implicates TRAP1 as a mitochondrial matrix protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Heat shock protein 75 kDa, mitochondrial; Heat shock protein family C member 5; HSP 75; testicular tissue protein Li 209; TNF receptor-associated protein 1; TNFR-associated protein 1; TRAP-1; tumor necrosis factor type 1 receptor associated protein; Tumor necrosis factor type 1 receptor-associated protein
Gene Aliases: 2410002K23Rik; HSP 75; HSP75; HSP90L; HSPC5; TRAP-1; TRAP1
UniProt ID: (Human) Q12931, (Rat) Q5XHZ0, (Mouse) Q9CQN1
Entrez Gene ID: (Human) 10131, (Rat) 287069, (Mouse) 68015
protein folding
negative regulation of cellular respiration
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
response to stress
translational attenuation
chaperone-mediated protein folding
negative regulation of reactive oxygen species biosynthetic process
negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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