Invitrogen

TRAP1 Monoclonal Antibody (TRAP1-6)

TRAP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

TRAP1 Antibody (MA1-010) in ICC/IF

Immunofluorescent analysis of TRAP1 in NCI-H460 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a TRAP1 monoclonal antibody (Product # MA1-010) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). TRAP1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

TRAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TRAP1 Antibody in Immunoprecipitation (IP)

TRAP1 Antibody in Western Blot, Flow Cytometry (WB, Flow)

Product Image Gallery

TRAP1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with TRAP1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-TRAP1 Monoclonal Antibody (TRAP1-6) (Product # MA1-010). {KD}

Product Details

MA1-010

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunohistochemistry (Frozen) (IHC (F))

1:20

Immunocytochemistry (ICC/IF)

1:250

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

TRAP1-6

Immunogen

Purified recombinant TRAP1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2207987

Product Specific Information

MA1-010 detects tumor necrosis factor receptor-associated protein (TRAP1) from human tissues.

MA1-010 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~75 kDa protein representing TRAP1. Immunofluorescence staining of TRAP1 in PC-3-M cells with MA1-010 produces a pattern consistent with mitochondrial staining. Immunoprecipitation of TRAP1 using MA1-010 fails to co-precipitate p23, Hop, or CyP40 suggesting TRAP1’s inability to associate with these co-chaperones.

The MA1-010 immunogen is purified, recombinant, human TRAP1.

Target Information

The 90 kDa heat shock protein (hsp90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. Hsp90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of hsp90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like hsp90, TRAP1 is found to be associated with numerous proteins involved in diverse actions. Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting presequence strongly implicates TRAP1 as a mitochondrial matrix protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mitochondria are devoid of poly(ADP-ribose)polymerase-1, but harbor its product oligo(ADP-ribose).

Journal of cellular biochemistry

Köritzer J,Blenn C,Bürkle A,Beneke S

MA1-010 was used in Immunoprecipitation to investigate the potential presence of poly(ADP-ribose) polymerase-1 in mitochondria.

Sat May 01 00:00:00 UTC 2021

Mitochondria are devoid of poly(ADP-ribose)polymerase-1, but harbor its product oligo(ADP-ribose).

Journal of cellular biochemistry

Köritzer J,Blenn C,Bürkle A,Beneke S

MA1-010 was used in Immunoprecipitation to investigate the potential presence of poly(ADP-ribose) polymerase-1 in mitochondria.

Sat May 01 00:00:00 UTC 2021

Cytotoxicity of withaferin A in glioblastomas involves induction of an oxidative stress-mediated heat shock response while altering Akt/mTOR and MAPK signaling pathways.

Investigational new drugs

Grogan PT,Sleder KD,Samadi AK,Zhang H,Timmermann BN,Cohen MS

MA1-010 was used in western blot to study the molecular mechanism of withaferin A cytotoxicity in glioblastomas

Sat Jun 01 00:00:00 UTC 2013

Cytotoxicity of withaferin A in glioblastomas involves induction of an oxidative stress-mediated heat shock response while altering Akt/mTOR and MAPK signaling pathways.

Investigational new drugs

Grogan PT,Sleder KD,Samadi AK,Zhang H,Timmermann BN,Cohen MS

MA1-010 was used in western blot to study the molecular mechanism of withaferin A cytotoxicity in glioblastomas

Sat Jun 01 00:00:00 UTC 2013

A novel C-terminal HSP90 inhibitor KU135 induces apoptosis and cell cycle arrest in melanoma cells.

Cancer letters

Samadi AK,Zhang X,Mukerji R,Donnelly AC,Blagg BS,Cohen MS

MA1-010 was used in western blot to study the effect of a C-terminal HSP90 inhibitor KU135 on melanoma cell death and proliferation

Thu Dec 22 00:00:00 UTC 2011

Involvement of tumor necrosis factor receptor-associated protein 1 (TRAP1) in apoptosis induced by beta-hydroxyisovalerylshikonin.

The Journal of biological chemistry

Masuda Y,Shima G,Aiuchi T,Horie M,Hori K,Nakajo S,Kajimoto S,Shibayama-Imazu T,Nakaya K

MA1-010 was used in western blot to study the role of mitochondrial TRAP1 expression in the ROS-induced apoptosis.

Fri Oct 08 00:00:00 UTC 2004

Hsp90 chaperone activity requires the full-length protein and interaction among its multiple domains.

The Journal of biological chemistry

Johnson BD,Chadli A,Felts SJ,Bouhouche I,Catelli MG,Toft DO

MA1-010 was used in immunoprecipitation to investigate the regulation of Hsp90 function in vitro and in vivo

Fri Oct 20 00:00:00 UTC 2000

Bioinformatics

Protein Aliases: Heat shock protein 75 kDa, mitochondrial; Heat shock protein family C member 5; HSP 75; testicular tissue protein Li 209; TNF receptor-associated protein 1; TNFR-associated protein 1; TRAP-1; Tumor necrosis factor type 1 receptor-associated protein

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Gene Aliases: HSP 75; HSP75; HSP90L; HSPC5; TRAP-1; TRAP1

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UniProt ID: (Human) Q12931

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Entrez Gene ID: (Human) 10131

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Function(s)

tumor necrosis factor receptor binding protein binding ATP binding protein kinase binding poly(A) RNA binding unfolded protein binding

Process(es)

response to stress translational attenuation chaperone-mediated protein folding negative regulation of cellular respiration negative regulation of reactive oxygen species biosynthetic process negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TRAP1 Monoclonal Antibody (TRAP1-6)

TRAP1 Antibody (MA1-010) in ICC/IF

Product Details

MA1-010

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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