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TRFP Antibody (PA5-79658) in WB
Western blot analysis of TRFP in Lane 1: rat brain tissue lysate, Lane 2: human PANC-1 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with TRFP polyclonal antibody (Product # PA5-79658) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by g... View More
Product Details
PA5-79658
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human TRFP / MED20 recombinant protein (Position: M1-R212).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746773
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, human PANC-1 whole cell.
Target Information
MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II, and general initiation factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hTRFP; Mediator complex subunit 20; Mediator of RNA polymerase II transcription subunit 20; Trf (TATA binding protein-related factor)-proximal homolog; Trf (TATA binding protein-related factor)-proximal protein homolog; TRF-proximal protein homolog; ubiquitin specific protease 49
Gene Aliases: 1110011O05Rik; 2410115I17Rik; AU018348; MED20; PRO0213; TRFP; Usp49
UniProt ID: (Human) Q9H944, (Mouse) Q9R0X0, (Rat) Q5XIE9
Entrez Gene ID: (Human) 9477, (Mouse) 56771, (Rat) 316209
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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