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Invitrogen
TRH Polyclonal Antibody
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FIGURE: 1 / 1
TRH Antibody (PA5-97006) in WB
Western blot analysis of TRH in extracts of various cell lines. Samples were incubated with TRH Polyclonal antibody (Product # PA5-97006) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-97006
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 25-230 of human TRH (NP_0090481)
Conjugate
Form
Liquid
Concentration
2.09 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808808
Product Specific Information
Positive Samples: SH-SY5Y, Mouse brain
Immunogen sequence: QPEAAQQEAV TAAEHPGLDD FLRQVERLLF LRENIQRLQG DQGEHSASQI FQSDWLSKRQ HPGKREEEEE EGVEEEEEEE GGAVGPHKRQ HPGRREDEAS WSVDVTQHKR QHPGRRSPWL AYAVPKRQHP GRRLADPKAQ RSWEEEEEEE EREEDLMPEK RQHPGKRALG GPCGPQGAYG QAGLLLGLLD DLSRSQGAEE KRQHPG
Target Information
This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Pro-thyrotropin-releasing hormone; Pro-TRH; Prothyroliberin; protirelin; thyrotropin releasing hormone; thyrotropin-releasing factor; thyrotropin-releasing hormone; TSH-releasing factor
Gene Aliases: Pro-TRH; TRF; TRH
UniProt ID: (Human) P20396, (Mouse) Q62361
Entrez Gene ID: (Human) 7200, (Mouse) 22044
response to hypoxia
histamine metabolic process
signal transduction
cell-cell signaling
adult walking behavior
response to cold
response to glucose
hormone-mediated signaling pathway
negative regulation of glutamate secretion
positive regulation of gamma-aminobutyric acid secretion
positive regulation of insulin secretion
eating behavior
response to ethanol
response to corticosterone
negative regulation of feeding behavior
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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