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TRHR Antibody (PA5-90309) in WB
Western blot analysis of TRHR in extracts of U-87MG cells. Samples were incubated with TRHR Polyclonal antibody (Product # PA5-90309) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
Product Details
PA5-90309
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 320-398 of human TRHR (NP_0032921)
Conjugate
Form
Liquid
Concentration
0.81 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806016
Product Specific Information
Immunogen sequence: YNLMSQKFRA AFRKLCNCKQ KPTEKPANYS VALNYSVIKE SDHFSTELDD ITVTDTYLSA TKVSFDDTCL ASEVSFSQS; Positive Samples: U-87MG; Cellular Location: Cell membrane, Multi-pass membrane protein
Target Information
TRH-R is a 398 amino acid integral membrane protein with six transmembrane domains and belongs to G-protein coupled receptor 1 family. It acts as a receptor for the signaling hormone TRH and activates the inositol phospholipid-calcium-protein kinase C transduction pathway upon binding with TRH. Reports suggest that TRH-R is a neurotransmitter and neuro regulator that controls dimerization of the TRH receptor and potentiates hormone-induced receptor trafficking. It is known to widely express in the thyrotroph cells of the anterior pituitary.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC141920; Thyroliberin receptor; Thyrotropin-releasing hormone receptor; TRH receptor; TRH-R; TRH1 Receptor
Gene Aliases: TRH-R; TRHR
UniProt ID: (Human) P34981
Entrez Gene ID: (Human) 7201
Performance Guarantee
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