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Invitrogen
TRIM Polyclonal Antibody
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TRIM Antibody (PA5-144018) in WB
Western blot analysis of TRAT1 using TRAT1 antibody (Product # PA5-144018). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates; Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane ... View More
Product Details
PA5-144018
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human TRAT1 recombinant protein (Position: S25-N186).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075232
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human MOLT-4 whole cell, human Jurkat whole cell. Flow: U251 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: pp29/30; T cell receptor interacting molecule; T-cell receptor-associated transmembrane adapter 1; T-cell receptor-interacting molecule; TRIM
Gene Aliases: HSPC062; pp29/30; TCRIM; TRAT1; TRIM
UniProt ID: (Human) Q6PIZ9
Entrez Gene ID: (Human) 50852
negative regulation of receptor recycling
adaptive immune response
cellular defense response
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
regulation of phosphatidylinositol 3-kinase signaling
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of calcium-mediated signaling
T cell receptor signaling pathway
positive regulation of T cell receptor signaling pathway
negative regulation of transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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