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Invitrogen
TRIM21 Polyclonal Antibody
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TRIM21 Antibody (PA5-114483) in IHC (P)
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using TRIM21 Polyclonal Antibody (Product # PA5-114483) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as ... View More
Product Details
PA5-114483
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-TRIM21 antibody was raised against a peptide corresponding to 16 amino acids near the center of human TRIM21. The immunogen is located within amino acids 290-340 of TRIM21.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2890362
Product Specific Information
Positive controls that may be used include: Human Lung Tissue Lysate, Human Small Intestine Tissue Slide, HeLa Cell Lysate, SK-N-SH Cell Lysate
Target Information
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro protein; E3 ubiquitin-protein ligase TRIM21; RING finger protein 81; Ro(SS-A); Sicca syndrome antigen A; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro); SS-A; tripartite motif-containing 21; Tripartite motif-containing protein 21
Gene Aliases: RNF81; Ro/SSA; RO52; SSA; SSA1; TRIM21
UniProt ID: (Human) P19474
Entrez Gene ID: (Human) 6737
protein polyubiquitination
protein monoubiquitination
cell cycle
positive regulation of autophagy
protein ubiquitination
protein destabilization
negative regulation of NF-kappaB transcription factor activity
regulation of type I interferon production
negative regulation of viral transcription
response to interferon-gamma
innate immune response
positive regulation of cell cycle
positive regulation of viral entry into host cell
positive regulation of sequence-specific DNA binding transcription factor activity
protein autoubiquitination
interferon-gamma-mediated signaling pathway
protein trimerization
negative regulation of protein deubiquitination
negative regulation of viral release from host cell
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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