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Invitrogen
TRIM21 Recombinant Rabbit Monoclonal Antibody (7Q6C3)
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FIGURE: 1 / 6
TRIM21 Antibody (MA5-51296) in WB
Western blot of TRIM21 was performed by loading 30 µg of A594 WT (lane 1), A594 TRIM21 KO (lane 2), PANC-1 (lane 3), SU.86.86 (lane 4), H4 (lane 5), 5637 (lane 6), BxPC-3 (lane 7), A-172 (lane 8), U-87 MG (lane 9) and FaDu (lane 10) cell lysates in RIPA buffer (Product # 89901) onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WXP42012BOX). Proteins on the blot were visualized with Ponceau staining (below immunoblot) (Product # BP10310). Proteins were transferred to nitrocellulose membrane and blocked in 5% milk for 1 hr. TRIM21 was ... View More
Product Details
MA5-51296
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7Q6C3
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 251-350 of human TRIM21/SS-A(NP_003132.2).
Conjugate
Form
Liquid
Concentration
0.29 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3093094
Product Specific Information
Immunogen sequence: LERSESWNLK DLDITSPELR SVCHVPGLKK MLRTCAVHIT LDPDTANPWL ILSEDRRQVR LGDTQQSIPG NEERFDSYPM VLGAQHFHSG KHYWEVDVTG
Target Information
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro protein; E3 ubiquitin-protein ligase TRIM21; RING finger protein 81; Ro(SS-A); Sicca syndrome antigen A; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro); SS-A; tripartite motif-containing 21; Tripartite motif-containing protein 21
Gene Aliases: RNF81; Ro/SSA; RO52; SSA; SSA1; TRIM21
UniProt ID: (Human) P19474
Entrez Gene ID: (Human) 6737
protein polyubiquitination
protein monoubiquitination
cell cycle
positive regulation of autophagy
protein ubiquitination
protein destabilization
negative regulation of NF-kappaB transcription factor activity
regulation of type I interferon production
negative regulation of viral transcription
response to interferon-gamma
innate immune response
positive regulation of cell cycle
positive regulation of viral entry into host cell
positive regulation of sequence-specific DNA binding transcription factor activity
protein autoubiquitination
interferon-gamma-mediated signaling pathway
protein trimerization
negative regulation of protein deubiquitination
negative regulation of viral release from host cell
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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