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Invitrogen
TRIM21 Recombinant Rabbit Monoclonal Antibody (PSH02-56)
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FIGURE: 1 / 11
TRIM21 Antibody (MA5-52082) in ICC/IF
Immunocytochemistry analysis of TRIM21 using U-2 OS cells. The cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. The cells were labeled with TRIM21 Recombinant Rabbit Monoclonal (PSH02-56) (Product # MA5-52082) (green)and beta tubulin (red) at 1:100 dilution in 1% BSA in PBST overnight at 4 degrees Celsius and then with iFluor™ 488 Goat Anti-Rabbit IgG H... View More
Product Details
MA5-52082
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH02-56
Immunogen
Recombinant protein within human TRIM21 aa 1-475.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3099396
Product Specific Information
Tissue Specificity: fetal and adult heart and fetal lung.
Positive Control: HeLa cell lysate, HeLa treated with 10 ng/mL IFN-alpha for 16 hours cell lysate, A549 cell lysate, 293T cell lysate, U-2 OS cell lysate, U-2 OS, human spleen tissue, human tonsil tissue.
Subcellular Location: Cytoplasm, Cytoplasmic vesicle, autophagosome, Nucleus, P-body.
Predicted band size: 54 kDa.
Target Information
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro protein; E3 ubiquitin-protein ligase TRIM21; RING finger protein 81; Ro(SS-A); Sicca syndrome antigen A; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro); SS-A; tripartite motif-containing 21; Tripartite motif-containing protein 21
Gene Aliases: RNF81; Ro/SSA; RO52; SSA; SSA1; TRIM21
UniProt ID: (Human) P19474
Entrez Gene ID: (Human) 6737
protein polyubiquitination
protein monoubiquitination
cell cycle
positive regulation of autophagy
protein ubiquitination
protein destabilization
negative regulation of NF-kappaB transcription factor activity
regulation of type I interferon production
negative regulation of viral transcription
response to interferon-gamma
innate immune response
positive regulation of cell cycle
positive regulation of viral entry into host cell
positive regulation of sequence-specific DNA binding transcription factor activity
protein autoubiquitination
interferon-gamma-mediated signaling pathway
protein trimerization
negative regulation of protein deubiquitination
negative regulation of viral release from host cell
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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