Proteintech

TRIM32 Polyclonal Antibody

View all (10) TRIM32 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (6)

TRIM32 Antibody in Immunocytochemistry (ICC/IF)

TRIM32 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

TRIM32 Antibody (10326-1-AP) in ICC/IF

IF result (cytoplasm stain) of anti-BBS11 (10326-1-AP; 1:50) with hTERT-RPE1 cell by Dr. Moshe Kim.

TRIM32 Antibody in Immunocytochemistry (ICC/IF)

TRIM32 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TRIM32 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TRIM32 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TRIM32 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

TRIM32 Antibody in Western Blot (WB)

Product Image Gallery

TRIM32 Antibody in Immunocytochemistry (ICC/IF) — IF result (cytoplasm stain) of anti-BBS11 (10326-1-AP; 1:50) with hTERT-RPE1 cell by Dr. Moshe Kim.

TRIM32 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lymphoma using 10326-1-AP (TRIM32 antibody) at dilution of 1:50 (under 10x lens).

TRIM32 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon using 10326-1-AP (TRIM32 antibody) at dilution of 1:25 (under 40x lens).

TRIM32 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon using 10326-1-AP (TRIM32 antibody) at dilution of 1:25 (under 10x lens).

TRIM32 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of ( 4% PFA) fixed mouse embryo tissue using 10326-1-AP (TRIM32 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

TRIM32 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

10326-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:20-1:200

View 3 publications 3 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TRIM32 Fusion Protein Ag0401 (111-430 aa encoded by BC003154)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: FCRSCGLVL CEPCREADHQ PPGHCTLPVK EAAEERRRDF GEKLTRLREL MGELQRRKAA LEGVSKDLQA RYKAVLQEYG HEERRVQDEL ARSRKFFTGS LAEVEKSNSQ VVEEQSYLLN IAEVQAVSRC DYFLAKIKQA DVALLEETAD EEEPELTASL PRELTLQDVE LLKVGHVGPL QIGQAVKKPR TVNVEDSWAM EATASAASTS VTFREMDMSP EEVVASPRAS PAKQRGPEAA SNIQQCLFLK KMGAKGSTPG MFNLPVSLYV TSQGEVLVAD RGNYRIQVFT RKGFLKEIRR SPSGIDSFVL SFLGADLPNL T (111-430 aa encoded by BC003154)

Target Information

TRIM32 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM32 localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ubiquitin ligase TRIM32 promotes dendrite arborization by mediating degradation of the epigenetic factor CDYL.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Liu L,Liu TT,Xie GG,Zhu XQ,Wang Y

Sat Jan 01 00:00:00 UTC 2022

Ubiquitin ligase TRIM32 promotes dendrite arborization by mediating degradation of the epigenetic factor CDYL.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Liu L,Liu TT,Xie GG,Zhu XQ,Wang Y

Sat Jan 01 00:00:00 UTC 2022

Ubiquitin ligase TRIM32 promotes dendrite arborization by mediating degradation of the epigenetic factor CDYL.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Liu L,Liu TT,Xie GG,Zhu XQ,Wang Y

Sat Jan 01 00:00:00 UTC 2022

Ubiquitin ligase TRIM32 promotes dendrite arborization by mediating degradation of the epigenetic factor CDYL.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Liu L,Liu TT,Xie GG,Zhu XQ,Wang Y

Sat Jan 01 00:00:00 UTC 2022

Ubiquitin ligase TRIM32 promotes dendrite arborization by mediating degradation of the epigenetic factor CDYL.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Liu L,Liu TT,Xie GG,Zhu XQ,Wang Y

Sat Jan 01 00:00:00 UTC 2022

Ubiquitin ligase TRIM32 promotes dendrite arborization by mediating degradation of the epigenetic factor CDYL.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Liu L,Liu TT,Xie GG,Zhu XQ,Wang Y

Sat Jan 01 00:00:00 UTC 2022

Deficiency of TRIM32 Impairs Motor Function and Purkinje Cells in Mid-Aged Mice.

Frontiers in aging neuroscience

Zhu JW,Jia WQ,Zhou H,Li YF,Zou MM,Wang ZT,Wu BS,Xu RX

Tue Aug 24 00:00:00 UTC 2021

A nonautophagic role of ATG5 in regulating cell growth by targeting c-Myc for proteasome-mediated degradation.

iScience

Li S,Zhang L,Zhang G,Shangguan G,Hou X,Duan W,Xi Y,Xu N,Zhang B,Dong J,Wang Y,Cui W,Chen S

Fri Nov 19 00:00:00 UTC 2021

Knockdown of TRIM32 inhibits tumor growth and increases the therapeutic sensitivity to temozolomide in glioma in a p53-dependent and -independent manner.

Biochemical and biophysical research communications

Cai Y,Gu WT,Cheng K,Jia PF,Li F,Wang M,Zhang WF,Qiu JT,Wu ZB,Zhao WG

Fri Apr 23 00:00:00 UTC 2021

Knockdown of TRIM32 inhibits tumor growth and increases the therapeutic sensitivity to temozolomide in glioma in a p53-dependent and -independent manner.

Biochemical and biophysical research communications

Cai Y,Gu WT,Cheng K,Jia PF,Li F,Wang M,Zhang WF,Qiu JT,Wu ZB,Zhao WG

Fri Apr 23 00:00:00 UTC 2021

Generation of the short TRIM32 isoform is regulated by Lys 247 acetylation and a PEST sequence.

PloS one

Garcia-Garcia J,Overå KS,Khan W,Sjøttem E

Fri Oct 15 00:00:00 UTC 2021

An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.

Science advances

Wilson MM,Callens C,Le Gallo M,Mironov S,Ding Q,Salamagnon A,Chavarria TE,Viel R,Peasah AD,Bhutkar A,Martin S,Godey F,Tas P,Kang HS,Juin PP,Jetten AM,Visvader JE,Weinberg RA,Attanasio M,Prigent C,Lees JA,Guen VJ

Fri Oct 29 00:00:00 UTC 2021

Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis.

Journal of the American Society of Nephrology : JASN

Koehler S,Kuczkowski A,Kuehne L,Jüngst C,Hoehne M,Grahammer F,Eddy S,Kretzler M,Beck BB,Höhfeld J,Schermer B,Benzing T,Brinkkoetter PT,Rinschen MM

Sun Mar 01 00:00:00 UTC 2020

TRIM32, but not its muscular dystrophy-associated mutant, positively regulates and is targeted to autophagic degradation by p62/SQSTM1.

Journal of cell science

Overå KS,Garcia-Garcia J,Bhujabal Z,Jain A,Øvervatn A,Larsen KB,Deretic V,Johansen T,Lamark T,Sjøttem E

Mon Dec 02 00:00:00 UTC 2019

TRIM32 promotes cell proliferation and invasion by activating β-catenin signalling in gastric cancer.

Journal of cellular and molecular medicine

Wang C,Xu J,Fu H,Zhang Y,Zhang X,Yang D,Zhu Z,Wei Z,Hu Z,Yan R,Cai Q

Mon Oct 01 00:00:00 UTC 2018

TRIM32 promotes cell proliferation and invasion by activating β-catenin signalling in gastric cancer.

Journal of cellular and molecular medicine

Wang C,Xu J,Fu H,Zhang Y,Zhang X,Yang D,Zhu Z,Wei Z,Hu Z,Yan R,Cai Q

Mon Oct 01 00:00:00 UTC 2018

TRIM32 promotes cell proliferation and invasion by activating β-catenin signalling in gastric cancer.

Journal of cellular and molecular medicine

Wang C,Xu J,Fu H,Zhang Y,Zhang X,Yang D,Zhu Z,Wei Z,Hu Z,Yan R,Cai Q

Mon Oct 01 00:00:00 UTC 2018

TRIM32 promotes cell proliferation and invasion by activating β-catenin signalling in gastric cancer.

Journal of cellular and molecular medicine

Wang C,Xu J,Fu H,Zhang Y,Zhang X,Yang D,Zhu Z,Wei Z,Hu Z,Yan R,Cai Q

Mon Oct 01 00:00:00 UTC 2018

Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component.

Human molecular genetics

Kudryashova E,Wu J,Havton LA,Spencer MJ

Wed Apr 01 00:00:00 UTC 2009

Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component.

Human molecular genetics

Kudryashova E,Wu J,Havton LA,Spencer MJ

Wed Apr 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: 72 kDa Tat-interacting protein; bM468K2.2 (tripartite motif protein 32); E3 ubiquitin-protein ligase TRIM32; RING-type E3 ubiquitin transferase TRIM32; RP11-67K19.1; TAT-interactive protein, 72-KD; tripartite motif protein 32; tripartite motif-containing 32; Tripartite motif-containing protein 32; zinc finger protein 117; Zinc finger protein HT2A; zinc-finger protein HT2A

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Gene Aliases: 1810045E12Rik; 3f3; BBS11; HT2A; LGMD2H; TATIP; TRIM32; Zfp117

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UniProt ID: (Human) Q13049, (Mouse) Q8CH72

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Entrez Gene ID: (Human) 22954, (Mouse) 69807, (Rat) 313264

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transcription coactivator activity RNA binding ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity myosin binding Tat protein binding translation initiation factor binding ubiquitin binding protein self-association ubiquitin protein ligase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity molecular_function

protein polyubiquitination tissue homeostasis actin ubiquitination response to UV protein ubiquitination positive regulation of cell growth positive regulation of cell migration regulation of type I interferon production negative regulation of viral transcription response to tumor necrosis factor protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response fat cell differentiation positive regulation of neuron differentiation positive regulation of protein catabolic process positive regulation of cell cycle positive regulation of proteolysis muscle cell cellular homeostasis negative regulation of fibroblast proliferation positive regulation of neurogenesis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of striated muscle cell differentiation axon development negative regulation of viral release from host cell negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage positive regulation of tumor necrosis factor-mediated signaling pathway positive regulation of interleukin-17-mediated signaling pathway positive regulation of chemokine (C-C motif) ligand 20 production positive regulation of cell motility negative regulation of keratinocyte apoptotic process biological_process

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