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Invitrogen
TRIM5 alpha/gamma/delta Polyclonal Antibody
FIGURE: 1 / 4
TRIM5 alpha/gamma/delta Antibody (PA5-18156) in IHC (P)
Immunohistochemistry analysis of TRIM5 alpha/gamma/delta in human uterus. Samples were incubated with TRIM5 alpha/gamma/delta polyclonal antibody (Product # PA5-18156) using a dilution of 5 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Product Details
PA5-18156
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (ASGILVNVKEEVTC) corresponding to the N-terminus amino acids of TRIM5 (aa 2-15).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10974151
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
Target Information
TRIM5a is a 493 amino acid member of the large tripartite motif protein (TRIM) family. TRIM proteins are composed of three zinc-binding domains, a RING, a B-box 2 and a coiled-coil domain, and they use homomultimerization to identify different cell compartments. Some TRIM proteins, such as TRIM5a, also possess a carboxy-terminal B30.2 (SPRY) domain and localize to the cytoplasm. TRIM5a mediates innate intracellular retroviral resistance, which is dependent on its carboxy-terminal domain. The three variable regions of the B30.2 domain form loops on one side of the B30.2 core structure of TRIM5a which may form a binding surface for the virus. TRIM5a trimerization plays a major role in its affinity for the retroviral capsid, and in its ability to inhibit virus infection. The linker region between the coiled-coil and B30.2 domains of TRIM5a is required for this trimerization. TRIM5a blocks infection after the virus has entered the cell.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RING finger protein 88; RING-type E3 ubiquitin transferase TRIM5; trim5a; tripartite motif containing 5 transcript variant iota; tripartite motif containing 5 transcript variant kappa; tripartite motif protein TRIM5; Tripartite motif-containing protein 5
Gene Aliases: RNF88; TRIM5; TRIM5alpha
UniProt ID: (Human) Q9C034
Entrez Gene ID: (Human) 85363
activation of innate immune response
pattern recognition receptor signaling pathway
autophagy
regulation of lipopolysaccharide-mediated signaling pathway
regulation of protein localization
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
innate immune response
negative regulation of viral entry into host cell
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of NF-kappaB transcription factor activity
defense response to virus
interferon-gamma-mediated signaling pathway
protein K63-linked ubiquitination
negative regulation of viral release from host cell
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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