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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TRIM59 Antibodies

          Invitrogen

          TRIM59 Polyclonal Antibody

          Advanced Verification
          View all (4) TRIM59 antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite TRIM59 Polyclonal Antibody

          • Antibody Testing Data (2)
          • Advanced Verification (1)
          TRIM59 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          TRIM59 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          TRIM59 Antibody (PA5-106512) in ICC/IF

          Immunofluorescence analysis of Tripartite motif-containing protein 59 was performed using 70% confluent log phase THP-1 differentiated to M0 macrophages treated with 1 µg/mL LPS for 24h. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TRIM59 Polyclonal Antibody (Product # PA5-106512) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          TRIM59 Antibody in Immunocytochemistry (ICC/IF)
          TRIM59 Antibody in Western Blot (WB)
          TRIM59 Antibody

          Product Details

          PA5-106512

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthesized peptide derived from human TRIM59(Accession Q8IWR1), corresponding to amino acid residues K201-L251.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2854181

          Product Specific Information

          Antibody detects endogenous levels of total TRIM59.

          Target Information

          May serve as a multifunctional regulator for innate immune signaling pathways. [UniProt]

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

          Have you cited this product in a publication?
          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: RING finger 1; RING finger protein 1; RING finger protein 104; tripartite motif-containing 57; tripartite motif-containing 59; Tripartite motif-containing protein 59; Tumor suppressor TSBF-1; tumor suppressor TSBF1; tumor suppressor TSBF1 homolog

          View more View less

          Gene Aliases: 2310035M22Rik; 2700022F13Rik; IFT80L; MRF1; RGD1311956; RNF104; TRIM57; TRIM59; TSBF1

          View more View less

          UniProt ID: (Human) Q8IWR1, (Mouse) Q922Y2

          View more View less

          Entrez Gene ID: (Human) 286827, (Mouse) 66949, (Rat) 365813

          View more View less

          Function(s)
          protein binding zinc ion binding metal ion binding ubiquitin protein ligase activity ubiquitin-protein transferase activity
          Process(es)
          protein ubiquitination negative regulation of I-kappaB kinase/NF-kappaB signaling innate immune response negative regulation of viral entry into host cell cilium assembly
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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