Invitrogen

TRIP Polyclonal Antibody

TRIP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TRIP Antibody (PA5-27699) in IHC (P)

Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using TRAIP (Product # PA5-27699) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

TRIP Antibody in Immunohistochemistry (IHC)

Product Image Gallery

TRIP Antibody — TRAIP is critical for RAP80 recruitment to the sites of DNA lesions. (a-c) U2OS cells expressing GFP-RAP80 or GFP-TRAIP were transfected with indicated siRNAs or siRNAs together with siRNA-resistant TRAIP WT, TRAIP-D1, -D2 or -D6. After 72 h, cells were subjected to laser microirradiation. Laser stripes were examined at the indicated time points. The intensity of each laser stripe in each time point was determined by averaging values from 10 cells and was graphed in the right panel. Experimental strategy is illustrated. (d) Cell fractionation to determine protein localization in response to DNA damage. The 293 T cells were transfected with control, RAP80 or TRAIP siRNA. After 48 h, the transfected cells were exposed to 0 or 10 Gy of ionizing radiation for 1 h. The chromatin fractions were subjected to western blot analysis. (e) Western blot analysis for TRAIP or RAP80 protein level in 293 T cells transfected with indicated siRNAs. (f) Kinetics of GFP-TRAIP or GFP-RAP80 translocation to DSBs. Average intensity of the laser stripes from 10 cells was presented with graph. (g,h) GFP-tagged RAP80 WT or indicated mutant was individually transfected into U2OS cells. After 72 h, the cells were subjected to laser microirradiation. The intensity of each laser stripe in each time point was determined by averaging values from 10 cells. Error bars indicate the s.d. See full blots in the Supplementary Fig. 14. {KD}

Product Details

PA5-27699

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRIP. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.9 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2545175

Product Specific Information

Recommended positive controls: A549.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TRAIP is a protein that contains an N-terminal RING finger motif and a putative coiled-coil domain. A similar murine protein interacts with TNFR-associated factor 1 (TRAF1), TNFR-associated factor 2 (TRAF2), and cylindromatosis. The interaction with TRAF2 inhibits TRAF2-mediated nuclear factor kappa-B, subunit 1 activation that is required for cell activation and protection against apoptosis.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TRAIP regulates Histone H2B monoubiquitination in DNA damage response pathways.

Oncology reports

Han YG,Yun M,Choi M,Lee SG,Kim H

PA5-27699 was used in Immunohistochemistry to demonstrate the clinical implication of H2B monoubiquitination and the potential role of tumor necrosis factor receptor-associated factor-interacting protein (TRAIP) in regulating its modification in lung adenocarcinoma.

Sat Jun 01 00:00:00 UTC 2019

TRAIP/RNF206 is required for recruitment of RAP80 to sites of DNA damage.

Nature communications

Soo Lee N,Jin Chung H,Kim HJ,Yun Lee S,Ji JH,Seo Y,Hun Han S,Choi M,Yun M,Lee SG,Myung K,Kim Y,Chul Kang H,Kim H

PA5-27699 was used in immunohistochemistry to report that the RAP80-binding partner TRAIP regulates recruitment of the damage signaling machinery and promotes homologous recombination

Tue Jan 19 00:00:00 UTC 2016

TRAIP/RNF206 is required for recruitment of RAP80 to sites of DNA damage.

Nature communications

Soo Lee N,Jin Chung H,Kim HJ,Yun Lee S,Ji JH,Seo Y,Hun Han S,Choi M,Yun M,Lee SG,Myung K,Kim Y,Chul Kang H,Kim H

PA5-27699 was used in immunohistochemistry to report that the RAP80-binding partner TRAIP regulates recruitment of the damage signaling machinery and promotes homologous recombination

Tue Jan 19 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase TRAIP; RING finger protein 206; TRAF-interacting protein

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Gene Aliases: RNF206; SCKL9; TRAIP; TRIP

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UniProt ID: (Human) Q9BWF2

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Entrez Gene ID: (Human) 10293

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Function(s)

receptor signaling protein activity protein binding zinc ion binding ligase activity ubiquitin protein ligase activity

Process(es)

apoptotic process signal transduction cell proliferation negative regulation of tumor necrosis factor-mediated signaling pathway protein ubiquitination negative regulation of interferon-beta production intracellular signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TRIP Polyclonal Antibody

TRIP Antibody (PA5-27699) in IHC (P)

Product Details

PA5-27699

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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