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TRIT1 Antibody (PA5-88984) in WB
Western blot analysis of TRIT1 in extracts of HeLa cells. Samples were incubated with TRIT1 Polyclonal antibody (Product # PA5-88984) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Product Details
PA5-88984
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 48-300 of human TRIT1 (NP_0601162)
Conjugate
Form
Liquid
Concentration
1.25 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805272
Product Specific Information
Immunogen sequence: GGEIVSADSM QVYEGLDIIT NKVSAQEQRI CRHHMISFVD PLVTNYTVVD FRNRATALIE DIFARDKIPI VVGGTNYYIE SLLWKVLVNT KPQEMGTEKV IDRKVELEKE DGLVLHKRLS QVDPEMAAKL HPHDKRKVAR SLQVFEETGI SHSEFLHRQH TEEGGGPLGG PLKFSNPCIL WLHADQAVLD ERLDKRVDDM LAAGLLEELR DFHRRYNQKN VSENSQDYQH GIFQSIGFKE FHEYLITEGK CTL; Positive Samples: HeLa; Cellular Location: Cytoplasm, Mitochondrion
Target Information
TRIT1 is responsible for the modification of A37 to isopentenyl A37 of both cytosolic and mitochondrial tRNAs.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hGRO1; IPP transferase; IPTase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; tRNA dimethylallyltransferase; tRNA isopentenylpyrophosphate transferase; tRNA isopentenyltransferase 1
Gene Aliases: GRO1; hGRO1; IPPT; IPT; IPTase; MOD5; TRIT1
UniProt ID: (Human) Q9H3H1
Entrez Gene ID: (Human) 54802
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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