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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TRITC Antibodies

          Invitrogen

          TRITC Polyclonal Antibody

          4 Published Figures
          64 References
          View all (4) TRITC antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite TRITC Polyclonal Antibody

          • Published Figures (4)
          TRITC Antibody in Immunohistochemistry (IHC)
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          TRITC Antibody in Immunohistochemistry (IHC)
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          FIGURE: 1 / 4

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          TRITC Antibody (A-6397) in IHC

          BMC genomics 2019 - Fig. 1 Mouse pyramidotomy model in neonates and adults. a - d , Representative images of immunohistochemistry for PKCgamma (a corticospinal tract (CST) marker) using the cervical cord of mice 4 weeks after injury. The dorsal column of sham ( a , c ) or pyramidotomy ( b , d ) groups, which were injured at P7 ( a , b ) or 8 weeks of age ( c , d ), are shown. White broken lines indicate the denervated side of ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          TRITC Antibody in Immunohistochemistry (IHC)
          TRITC Antibody in Immunohistochemistry (IHC)
          TRITC Antibody in Immunohistochemistry (IHC)
          TRITC Antibody in Immunohistochemistry (IHC)

          Product Details

          A-6397

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-dependent
          View 7 publications 7 publications

          Immunohistochemistry (IHC)

          Assay-dependent
          View 29 publications 29 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunohistochemistry - Free Floating (IHC (Free))

          -
          View 5 publications 5 publications

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          View 4 publications 4 publications

          Flow Cytometry (Flow)

          -
          View 3 publications 3 publications

          Immunoprecipitation (IP)

          -
          View 2 publications 2 publications

          Neutralization (Neu)

          -
          View 1 publication 1 publication

          Functional Assay (Functional)

          -
          View 2 publications 2 publications

          Affinity Purification (AP)

          -
          View 1 publication 1 publication

          In vitro Assay (IV)

          -
          View 2 publications 2 publications

          Miscellaneous PubMed (MISC)

          -
          View 7 publications 7 publications
          Product Specifications

          Species Reactivity

          Chemical

          Published species

          Chemical, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Tetramethylrhodamine

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          purified

          Storage buffer

          PBS, pH 7.2

          Contains

          5mM sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2536196

          Product Specific Information

          We offer a variety of anti-fluorescent dye antibodies that recognize specific fluorophores and, in most cases, quench their fluorescence. These anti-dye antibodies, including those that recognize the tetramethylrhodamine (TRITC) and Texas Red™ fluorophores, can also serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria, or other biomolecules have been internalized by endocytic or pinocytic processes. Our anti-tetramethylrhodamine (A6397) and anti-Texas Red (A6399) antibodies have been raised against the tetramethylrhodamine and Texas Red fluorophores, respectively. These antibodies quench much of the fluorescence of the complementary dyes. However, due to the related chemical structures, the anti-tetramethylrhodamine antibody crossreacts with the Texas Red fluorophore and the anti-Texas Red antibody cross-reacts with tetramethylrhodamine. Also, both antibodies tightly bind the Rhodamine Red™ fluorophore. Thus, either antibody can serve as an effective probe for tetramethylrhodamine, Texas Red, or Rhodamine Red dyes.

          We use a sensitive quenching assay to ensure that this antibody is provided at a consistently high titer value. As supplied, 20 µL of the antibody solution is certified to produce ≥50% of the maximal fluorescence quenching of 1 mL of a 50 nM solution of tetramethylrhodamine, assayed in 100 mM sodium phosphate, pH 8.0. Maximal quenching for tetramethylrhodamine is ~80% of the fluorescence of the free dye. Due to steric hindrance, maximal fluorescence quenching of the tetramethylrhodamine covalently bound to protein may be significantly less.

          Target Information

          Tetramethylrhodamine (TRITC) is a bright orange-fluorescent traditional dye with excitation ideally suited to the 532 nm laser line. It has been used extensively to label protein conjugates such as phalloidins for cytoskeleton counterstaining or antibody conjugates for immunofluorescence and other cellular imaging applications. Unlike biotin, which is an endogenous ligand in mitochondria, dye-based haptens permit background-free staining of cells and tissues and a sensitive alternative to biotin-streptavidin methods.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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