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Invitrogen
TRPC5 Polyclonal Antibody
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FIGURE: 1 / 1
TRPC5 Antibody (PA5-95221) in WB
Western blot analysis of TRPC5 using TRPC5 Polyclonal Antibody (Product # PA5-95221). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with TRPC5 Polyclonal Antibody at 1:200 overnight at 4°C, th... View More
Product Details
PA5-95221
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human TRPC5 recombinant protein (Position: K684-L973).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807025
Product Specific Information
Human TRPC5 shares 90% amino acid (aa) sequence identity with mouse TRPC5.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: HELA whole cell, U87 whole cell, COLO320 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
TRPC5 belongs to the transient receptor family. TRPC5 is one of the seven mammalian TRPC (transient receptor potential channel) proteins. The TRPC5 protein is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC5 is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4. TRPC5 also interacts with multiple proteins including calmodulin, CABP1, enkurin, Na(+)-H+ exchange regulatory factor (NHERF ), interferon-induced GTP-binding protein (MX1), ring finger protein 24 (RNF24), and SEC14 domain and spectrin repeat-containing protein 1 (SESTD1). TRPC5 is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC5 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, or G-protein coupled receptors. TRPC5 has also been shown to be calcium-selective, and may also be activated by intracellular calcium store depletion. Diseases associated with TRPC5 include Hypertrophic Pyloric Stenosis and Pyloric Stenosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Htrp-5; hTRP5; protein phosphatase 1, regulatory subunit 159; Short transient receptor potential channel 5; transient receptor potential cation channel, subfamily C, member 5; transient receptor potential channel 5; Transient receptor protein 5; TRP-5; TrpC5
Gene Aliases: PPP1R159; TRP5; TRPC5
UniProt ID: (Human) Q9UL62
Entrez Gene ID: (Human) 7224
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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