Proteintech

TRPC6 Polyclonal Antibody

TRPC6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TRPC6 Antibody (18236-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue slide using (Product # 18236-1-AP) TRPC6 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRPC6 Antibody in Immunoprecipitation (IP)

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Product Details

18236-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TRPC6 Fusion Protein Ag12976 (1-405 aa encoded by BC093658)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSQSPAFGP RRGSSPRGAA GAAARRNESQ DYLLMDSELG EDGCPQAPLP CYGYYPCFRG SDNRLAHRRQ TVLREKGRRL ANRGPAYMFS DRSTSLSIEE ERFLDAAEYG NIPVVRKMLE ECHSLNVNCV DYMGQNALQL AVANEHLEIT ELLLKKENLS RVGDALLLAI SKGYVRIVEA ILSHPAFAEG KRLATSPSQS ELQQDDFYAY DEDGTRFSHD VTPIILAAHC QEYEIVHTLL RKGARIERPH DYFCKCNDCN QKQKHDSFSH SRSRINAYKG LASPAYLSLS SEDPVMTALE LSNELAVLAN IEKEFKNDYK KLSMQCKDFV VGLLDLCRNT EEVEAILNGD VETLQSGDHG RPNLSRLKLA IKYEVKKFVA HPNCQQQLLS IWYENLSGLR QQTMAV (1-405 aa encoded by B C093658)

Target Information

Trpc6 forms a receptor-activated calcium channel in the cell membrane. The Trpc6 channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of the Trpc6 channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in the Trpc6 gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). Further, Trpc6 is a member of the mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the ″canonical TRP″ (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC6/6/7 subfamily can be activated by diacylglycerol (DAG) analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. When expressed in transfected cells, Trpc6 acts as a non-selective store-independent receptor-activated cation channel. Trpc6 is activated by DAG in a PKC-independent manner and is insensitive to IP3 activation. There is increasing evidence that Trpc6 encodes endogenous DAG-activated receptor-operated cation channels in vivo. Diseases associated with TRPC6 include Glomerulosclerosis, Focal Segmental, 2 and Focal Segmental Glomerulosclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Ai Z,Wang M,Zhou Y,Yuan D,Jian Q,Wu S,Liu B,Yang Y

Mon May 01 00:00:00 UTC 2023

TRPC6 interacted with K_Ca1.1 channels to regulate the proliferation and apoptosis of glioma cells.

Archives of biochemistry and biophysics

Yin H,Cheng H,Li P,Yang Z

Mon Aug 15 00:00:00 UTC 2022

TRPC6 interacted with K_Ca1.1 channels to regulate the proliferation and apoptosis of glioma cells.

Archives of biochemistry and biophysics

Yin H,Cheng H,Li P,Yang Z

Mon Aug 15 00:00:00 UTC 2022

TRPC6 interacted with K_Ca1.1 channels to regulate the proliferation and apoptosis of glioma cells.

Archives of biochemistry and biophysics

Yin H,Cheng H,Li P,Yang Z

Mon Aug 15 00:00:00 UTC 2022

Dexmedetomidine Alleviates Neuropathic Pain via the TRPC6-p38 MAPK Pathway in the Dorsal Root Ganglia of Rats.

Journal of pain research

Xu S,Yi Y,Wang Y,Wang P,Zhao Y,Feng W

Wed Nov 01 00:00:00 UTC 2023

Sildenafil protects against pulmonary hypertension induced by hypoxia in neonatal rats via activation of PPARγ‑mediated downregulation of TRPC.

International journal of molecular medicine

Huang W,Liu N,Tong X,Du Y

Tue Feb 01 00:00:00 UTC 2022

Sildenafil protects against pulmonary hypertension induced by hypoxia in neonatal rats via activation of PPARγ‑mediated downregulation of TRPC.

International journal of molecular medicine

Huang W,Liu N,Tong X,Du Y

Tue Feb 01 00:00:00 UTC 2022

Amelioration of circadian disruption and calcium-handling protein defects by choline alleviates cardiac remodeling in abdominal aorta coarctation rats.

Laboratory investigation; a journal of technical methods and pathology

He X,Yang S,Deng J,Wu Q,Zang WJ

Thu Jul 01 00:00:00 UTC 2021

Regenerative Metaplastic Clones in COPD Lung Drive Inflammation and Fibrosis.

Cell

Rao W,Wang S,Duleba M,Niroula S,Goller K,Xie J,Mahalingam R,Neupane R,Liew AA,Vincent M,Okuda K,O'Neal WK,Boucher RC,Dickey BF,Wechsler ME,Ibrahim O,Engelhardt JF,Mertens TCJ,Wang W,Jyothula SSK,Crum CP,Karmouty-Quintana H,Parekh KR,Metersky ML,McKeon FD,Xian W

Thu May 14 00:00:00 UTC 2020

Nongenetic optical modulation of neural stem cell proliferation and neuronal/glial differentiation.

Biomaterials

Wang M,Xu Z,Liu Q,Sun W,Jiang B,Yang K,Li J,Gong Y,Liu Q,Liu D,Li X

Sun Dec 01 00:00:00 UTC 2019

Nongenetic optical modulation of neural stem cell proliferation and neuronal/glial differentiation.

Biomaterials

Wang M,Xu Z,Liu Q,Sun W,Jiang B,Yang K,Li J,Gong Y,Liu Q,Liu D,Li X

Sun Dec 01 00:00:00 UTC 2019

Catalpol alleviates adriamycin-induced nephropathy by activating the SIRT1 signalling pathway in vivo and in vitro.

British journal of pharmacology

Zhang J,Bi R,Meng Q,Wang C,Huo X,Liu Z,Wang C,Sun P,Sun H,Ma X,Wu J,Liu K

Sun Dec 01 00:00:00 UTC 2019

TRPC6 channel translocation into phagosomal membrane augments phagosomal function.

Proceedings of the National Academy of Sciences of the United States of America

Riazanski V,Gabdoulkhakova AG,Boynton LS,Eguchi RR,Deriy LV,Hogarth DK,Loaëc N,Oumata N,Galons H,Brown ME,Shevchenko P,Gallan AJ,Yoo SG,Naren AP,Villereal ML,Beacham DW,Bindokas VP,Birnbaumer L,Meijer L,Nelson DJ

Tue Nov 24 00:00:00 UTC 2015

TRPC6 channel translocation into phagosomal membrane augments phagosomal function.

Proceedings of the National Academy of Sciences of the United States of America

Tue Nov 24 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Calcium entry channel; FLJ11098; FLJ14863; focal segmental glomerulosclerosis 2; HGNC:12338; Short transient receptor potential channel 6; transient receptor potential cation channel, subfamily C, member 6; Transient receptor protein 6; TRP-6; trp6A; TrpC6

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Gene Aliases: AV025995; FSGS2; LLHWJM002; LLHWJM003; LLHWJM004; mtrp6; TRP-6; TRP6; TRPC6; Trrp6

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UniProt ID: (Human) Q9Y210, (Mouse) Q61143

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Entrez Gene ID: (Human) 7225, (Mouse) 22068

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Function(s)

actin binding calcium channel activity protein binding store-operated calcium channel activity clathrin binding actinin binding ATPase binding inositol 1,4,5 trisphosphate binding ion channel activity cation channel activity

Process(es)

transport ion transport calcium ion transport manganese ion transport positive regulation of cytosolic calcium ion concentration positive regulation of ion transmembrane transporter activity positive regulation of calcium ion transport transmembrane transport cellular response to hydrogen peroxide calcium ion transmembrane transport cation transport single fertilization aging positive regulation of peptidyl-threonine phosphorylation platelet activation neuron differentiation positive regulation of neuron differentiation negative regulation of dendrite morphogenesis regulation of cytosolic calcium ion concentration cellular response to hypoxia

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TRPC6 Polyclonal Antibody

TRPC6 Antibody (18236-1-AP) in IHC (P)

Product Details

18236-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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