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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TRPC7 Antibodies

          Invitrogen

          TRPC7 Monoclonal Antibody (N64A/36), PerCP

          View all (10) TRPC7 antibodies

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          Datasheet
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          Datasheet
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          Cite TRPC7 Monoclonal Antibody (N64A/36), PerCP

          • Antibody Testing Data (4)
          TRPC7 Antibody in Immunocytochemistry (ICC/IF)
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          TRPC7 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          TRPC7 Antibody (MA5-45475) in ICC/IF

          Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with TrpC7 monoclonal antibody (Product # MA5-45475) at 1:100 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpC7 antibody (D) Composite. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          TRPC7 Antibody in Immunocytochemistry (ICC/IF)
          TRPC7 Antibody in Immunocytochemistry (ICC/IF)
          TRPC7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TRPC7 Antibody in Western Blot (WB)

          Product Details

          MA5-45475

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100
          -

          Immunocytochemistry (ICC/IF)

          1:100
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -

          Antibody Microarray (AM)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          N64A/36

          Immunogen

          Synthetic peptide amino acids 845-862 of human TRPC7
          View immunogen

          Conjugate

          PerCP PerCP PerCP
          • Unconjugated
          • APC 
          • FITC
          • PE 
          • Request custom conjugation

          Excitation/Emission Max

          482/675 nm View spectra spectra

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein G

          Storage buffer

          95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2931929

          Product Specific Information

          1 µg/mL of MA5-45475 was sufficient for detection of TrpC7 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.| Detects approximately 100kDa.

          Target Information

          TRPC7 is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC7 is probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. TRPC7 is also activated by diacylglycerol (DAG). Further, TRPC7 may also be activated by intracellular calcium store depletion.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: capacitative calcium channel TRPC7; hTRP7; likley ortholog of mouse transient receptor potential cation channel, subfamily C, member 7; mTRP7; putative capacitative calcium channel; Short transient receptor potential channel 7; transient receptor potential cation channel, subfamily C, member 7; Transient receptor protein 7; TRP-7; TRP7 protein; TrpC7

          View more View less

          Gene Aliases: TRP7; TRPC7; Trrp8

          View more View less

          UniProt ID: (Human) Q9HCX4, (Mouse) Q9WVC5

          View more View less

          Entrez Gene ID: (Human) 57113, (Rat) 282822, (Mouse) 26946

          View more View less

          Function(s)
          calcium channel activity protein binding store-operated calcium channel activity cation channel activity
          Process(es)
          manganese ion transport single fertilization platelet activation regulation of cytosolic calcium ion concentration calcium ion transmembrane transport calcium ion transport
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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