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TRPM8 Antibody (CF811228) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRPM8 (RC220615, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-TRPM8. Positive lysates LY402977 (100 µg) and LC402977 (20 µg) can be purchased separately from OriGene.
Product Details
CF811228
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI7A11
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-300 of human TRPM8 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
TRPM8 is a receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. TRPM8 is activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH and involved in menthol sensation. TRPM8 is permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. TRPM8 has an expression pattern within normal human tissues that is mostly restricted to prostate epithelial cells. TRPM8 mRNA expression is at moderate levels in normal prostate tissue and appears to be elevated in prostate cancer. Notably, TRPM8 mRNA was also expressed in a number of nonprostatic primary tumors of breast, colon, lung, and skin origin, whereas transcripts encoding TRPM8 were hardly detected or not detected in the corresponding normal human tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cold menthol receptor 1; cold/menthol receptor 1; Long transient receptor potential channel 6; LTrpC-6; MGC2849; short form of the TRPM8 cationic channel; transient receptor melastatin 8 variant 1; transient receptor melastatin 8 variant 2; Transient receptor potential cation channel subfamily M member 8; transient receptor potential cation channel, subfamily M, member 8; Transient receptor potential p8; transient receptor potential subfamily M member 8; transient receptor potential-p8; TRP ion channel TRPM8; Trp-p8
Gene Aliases: CMR1; LTrpC-6; LTRPC6; Trp-p8; TRPM8; TRPP8
UniProt ID: (Human) Q7Z2W7, (Rat) Q8R455, (Mouse) Q8R4D5
Entrez Gene ID: (Human) 79054, (Rat) 171384, (Mouse) 171382
calcium ion transport
cellular calcium ion homeostasis
response to temperature stimulus
response to cold
detection of temperature stimulus
thermoception
protein homotetramerization
protein homotrimerization
calcium ion transmembrane transport
transport
ion transport
cation transport
ion transmembrane transport
response to stimulus
sensory perception of temperature stimulus
transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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