Invitrogen

TSC1 Monoclonal Antibody (5C8A12)

TSC1 Antibody in Immunoprecipitation (IP)

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TSC1 Antibody (37-0400) in IP

Immunoprecipitation of TSC1 was performed on HAP1 cell lysates. Antibody-bead conjugates were prepared by adding 2 µg of TSC1 monoclonal antibody (Product # 370400) with 30 µL of protein G-Sepharose beads and rocked overnight at 4°C. 1 mg of lysate was incubated with an antibody-bead conjugate for 2 hours at 4°C. Following centrifugation and multiple washes, 10% starting material (SM), 10% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using a different antibody (Product # PA5-116079). Ponceau stai... View More

TSC1 Antibody in Immunocytochemistry (ICC/IF)

TSC1 Antibody in Immunohistochemistry (IHC)

TSC1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

TSC1 Antibody — Figure 5. YAP is required for the abnormal proliferation and survival of TSC1/2-null cells in vitro. (a and b) Expression of indicated proteins was analyzed by immunoblot in Tsc2+/+ and Tsc2−/− MEFs (these MEFs were generated in p53−/− background) cultured with or without serum for 24 h. Similar results were observed in three independent experiments. (c-e) Tsc2+/+ and Tsc2−/− MEFs transfected with siCtrl or siYAP were cultured in serum starvation conditions for a 72-h period. (c) Cell number was counted in different groups. Three independent experiments were performed and analyzed (Mean ± SEM; **, P < 0.01). (d) Expression of YAP protein was analyzed by immunoblot at the 24-h time point. Similar results were observed in three independent experiments. (e) Apoptotic cells were identified by TUNEL assay at the 48-h time point. The percentage of TUNEL+ cells was determined by counting TUNEL+ nuclei among at least 500 cells in distinct fields of each experiment. Three independent experiments were performed and analyzed. Mean ± SEM; *, P < 0.05, **, P < 0.01. (f and g) Tsc2+/+ and Tsc2−/− MEFs were cultured under serum starvation conditions and treated with DMSO or Verteporfin for a 72-h period. (f) Cell number was counted. Three independent experiments were performed and analyzed. Mean ± SEM; *, P < 0.05, **, P < 0.01. (g) Expression of Ctgf mRNA was assessed by qRT-PCR in different treatments. Three independent experiments were performed and analyzed. Mean ± SEM; *, P < 0.05, **, P < 0.01, ***, P < 0.001. (h) E {KD}

Product Details

37-0400

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5C8A12

Immunogen

Recombinant human Hamartin protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533292

Target Information

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MiR-130b modulates the invasive, migratory, and metastatic behavior of leiomyosarcoma.

PloS one

Danielson LS,Guijarro MV,Menendez S,Higgins B,Sun Q,Mittal K,Popiolek DA,Overholtzer M,Palmer GD,Hernando E

Published figure using TSC1 monoclonal antibody (Product # 37-0400) in Western Blot

Mon Jan 30 00:00:00 UTC 2023

G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling.

Cell

Prentzell MT,Rehbein U,Cadena Sandoval M,De Meulemeester AS,Baumeister R,Brohée L,Berdel B,Bockwoldt M,Carroll B,Chowdhury SR,von Deimling A,Demetriades C,Figlia G,de Araujo MEG,Heberle AM,Heiland I,Holzwarth B,Huber LA,Jaworski J,Kedra M,Kern K,Kopach A,Korolchuk VI,van 't Land-Kuper I,Macias M,Nellist M,Palm W,Pusch S,Ramos Pittol JM,Reil M,Reintjes A,Reuter F,Sampson JR,Scheldeman C,Siekierska A,Stefan E,Teleman AA,Thomas LE,Torres-Quesada O,Trump S,West HD,de Witte P,Woltering S,Yordanov TE,Zmorzynska J,Opitz CA,Thedieck K

37-0400 was used in Immunoprecipitation to report that Ras GTPase-activating protein-binding proteins 1 and 2 reside at the cytoplasmic surface of lysosomes.

Thu Feb 04 00:00:00 UTC 2021

G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling.

Cell

37-0400 was used in Immunoprecipitation to report that Ras GTPase-activating protein-binding proteins 1 and 2 reside at the cytoplasmic surface of lysosomes.

Thu Feb 04 00:00:00 UTC 2021

The E3 ubiquitin ligase Peli1 regulates the metabolic actions of mTORC1 to suppress antitumor T cell responses.

The EMBO journal

Ko CJ,Zhang L,Jie Z,Zhu L,Zhou X,Xie X,Gao T,Yang JY,Cheng X,Sun SC

37-0400 was used in Immunoprecipitation to establish Peli1 as a novel regulator of mTORC1 and downstream mTORC1-mediated actions on T cell metabolism and antitumor immunity.

Fri Jan 15 00:00:00 UTC 2021

MicroRNA-92b-3p is a prognostic oncomiR that targets TSC1 in clear cell renal cell carcinoma.

Cancer science

Wang C,Uemura M,Tomiyama E,Matsushita M,Koh Y,Nakano K,Hayashi Y,Ishizuya Y,Jingushi K,Kato T,Hatano K,Kawashima A,Ujike T,Nagahara A,Fujita K,Imamura R,Tsujikawa K,Nonomura N

37-0400 was used in Immunohistochemistry to investigate the role of miR-92b-3p in ccRCC.

Wed Apr 01 00:00:00 UTC 2020

MicroRNA-92b-3p is a prognostic oncomiR that targets TSC1 in clear cell renal cell carcinoma.

Cancer science

Wang C,Uemura M,Tomiyama E,Matsushita M,Koh Y,Nakano K,Hayashi Y,Ishizuya Y,Jingushi K,Kato T,Hatano K,Kawashima A,Ujike T,Nagahara A,Fujita K,Imamura R,Tsujikawa K,Nonomura N

37-0400 was used in Immunohistochemistry to investigate the role of miR-92b-3p in ccRCC.

Wed Apr 01 00:00:00 UTC 2020

miR-20b and miR-451a Are Involved in Gastric Carcinogenesis through the PI3K/AKT/mTOR Signaling Pathway: Data from Gastric Cancer Patients, Cell Lines and Ins-Gas Mouse Model.

International journal of molecular sciences

Streleckiene G,Inciuraite R,Juzenas S,Salteniene V,Steponaitiene R,Gyvyte U,Kiudelis G,Leja M,Ruzgys P,Satkauskas S,Kupcinskiene E,Franke S,Thon C,Link A,Kupcinskas J,Skieceviciene J

Published figure using TSC1 monoclonal antibody (Product # 37-0400) in Western Blot

Wed Jan 29 00:00:00 UTC 2020

R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein.

Nature communications

Cloutier P,Poitras C,Durand M,Hekmat O,Fiola-Masson É,Bouchard A,Faubert D,Chabot B,Coulombe B

Published figure using TSC1 monoclonal antibody (Product # 37-0400) in Western Blot

Wed May 31 00:00:00 UTC 2017

Pharmacological inhibition of Polo-like kinase 1 (PLK1) by BI-2536 decreases the viability and survival of hamartin and tuberin deficient cells via induction of apoptosis and attenuation of autophagy.

Cell cycle (Georgetown, Tex.)

Valianou M,Cox AM,Pichette B,Hartley S,Paladhi UR,Astrinidis A

37-0400 was used in western blot to elucidate how PLK1 regulates cell survival during mTORC1 hyperactivation.

Wed Dec 02 00:00:00 UTC 2015

Cell cycle (Georgetown, Tex.)

Valianou M,Cox AM,Pichette B,Hartley S,Paladhi UR,Astrinidis A

37-0400 was used in western blot to elucidate how PLK1 regulates cell survival during mTORC1 hyperactivation.

Wed Dec 02 00:00:00 UTC 2015

Cell cycle (Georgetown, Tex.)

Valianou M,Cox AM,Pichette B,Hartley S,Paladhi UR,Astrinidis A

37-0400 was used in western blot to elucidate how PLK1 regulates cell survival during mTORC1 hyperactivation.

Wed Dec 02 00:00:00 UTC 2015

Spatial control of the TSC complex integrates insulin and nutrient regulation of mTORC1 at the lysosome.

Cell

Menon S,Dibble CC,Talbott G,Hoxhaj G,Valvezan AJ,Takahashi H,Cantley LC,Manning BD

Published figure using TSC1 monoclonal antibody (Product # 37-0400) in Immunoprecipitation

Thu Feb 13 00:00:00 UTC 2014

Regulation of YAP by mTOR and autophagy reveals a therapeutic target of tuberous sclerosis complex.

The Journal of experimental medicine

Liang N,Zhang C,Dill P,Panasyuk G,Pion D,Koka V,Gallazzini M,Olson EN,Lam H,Henske EP,Dong Z,Apte U,Pallet N,Johnson RL,Terzi F,Kwiatkowski DJ,Scoazec JY,Martignoni G,Pende M

37-0400 was used in western blot to investigate if and how mTOR regulates YAP

Mon Oct 20 00:00:00 UTC 2014

Regulation of YAP by mTOR and autophagy reveals a therapeutic target of tuberous sclerosis complex.

The Journal of experimental medicine

Liang N,Zhang C,Dill P,Panasyuk G,Pion D,Koka V,Gallazzini M,Olson EN,Lam H,Henske EP,Dong Z,Apte U,Pallet N,Johnson RL,Terzi F,Kwiatkowski DJ,Scoazec JY,Martignoni G,Pende M

37-0400 was used in western blot to investigate if and how mTOR regulates YAP

Mon Oct 20 00:00:00 UTC 2014

TBC1D7 is a third subunit of the TSC1-TSC2 complex upstream of mTORC1.

Molecular cell

Dibble CC,Elis W,Menon S,Qin W,Klekota J,Asara JM,Finan PM,Kwiatkowski DJ,Murphy LO,Manning BD

Fri Aug 24 00:00:00 UTC 2012

TBC1D7 is a third subunit of the TSC1-TSC2 complex upstream of mTORC1.

Molecular cell

Dibble CC,Elis W,Menon S,Qin W,Klekota J,Asara JM,Finan PM,Kwiatkowski DJ,Murphy LO,Manning BD

Fri Aug 24 00:00:00 UTC 2012

Hepatitis B virus X protein upregulates mTOR signaling through IKKβ to increase cell proliferation and VEGF production in hepatocellular carcinoma.

PloS one

Yen CJ,Lin YJ,Yen CS,Tsai HW,Tsai TF,Chang KY,Huang WC,Lin PW,Chiang CW,Chang TT

37-0400 was used in western blot to study signaling pathways underlying HBx-induced liver tumorigenesis.

Thu Jan 10 00:00:00 UTC 2013

ARD1 stabilization of TSC2 suppresses tumorigenesis through the mTOR signaling pathway.

Science signaling

Kuo HP,Lee DF,Chen CT,Liu M,Chou CK,Lee HJ,Du Y,Xie X,Wei Y,Xia W,Weihua Z,Yang JY,Yen CJ,Huang TH,Tan M,Xing G,Zhao Y,Lin CH,Tsai SF,Fidler IJ,Hung MC

37-0400 was used in western blot to suggest ARD inhibits the mTOR pathway.

Tue Feb 09 00:00:00 UTC 2010

ARD1 stabilization of TSC2 suppresses tumorigenesis through the mTOR signaling pathway.

Science signaling

Kuo HP,Lee DF,Chen CT,Liu M,Chou CK,Lee HJ,Du Y,Xie X,Wei Y,Xia W,Weihua Z,Yang JY,Yen CJ,Huang TH,Tan M,Xing G,Zhao Y,Lin CH,Tsai SF,Fidler IJ,Hung MC

37-0400 was used in western blot to suggest ARD inhibits the mTOR pathway.

Tue Feb 09 00:00:00 UTC 2010

Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway.

Genes to cells : devoted to molecular & cellular mechanisms

Kume K,Iizumi Y,Shimada M,Ito Y,Kishi T,Yamaguchi Y,Handa H

Thu Apr 01 00:00:00 UTC 2010

Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway.

Genes to cells : devoted to molecular & cellular mechanisms

Kume K,Iizumi Y,Shimada M,Ito Y,Kishi T,Yamaguchi Y,Handa H

Thu Apr 01 00:00:00 UTC 2010

The tuberous sclerosis proteins regulate formation of the primary cilium via a rapamycin-insensitive and polycystin 1-independent pathway.

Human molecular genetics

Hartman TR,Liu D,Zilfou JT,Robb V,Morrison T,Watnick T,Henske EP

37-0400 was used in immunocytochemistry and western blot to characterize the ciliary disruption in tuberous sclerosis complex syndrome

Thu Jan 01 00:00:00 UTC 2009

The tuberous sclerosis proteins regulate formation of the primary cilium via a rapamycin-insensitive and polycystin 1-independent pathway.

Human molecular genetics

Hartman TR,Liu D,Zilfou JT,Robb V,Morrison T,Watnick T,Henske EP

37-0400 was used in immunocytochemistry and western blot to characterize the ciliary disruption in tuberous sclerosis complex syndrome

Thu Jan 01 00:00:00 UTC 2009

Clinicopathological and immunohistochemical findings in an autopsy case of tuberous sclerosis complex.

Neuropathology : official journal of the Japanese Society of Neuropathology

Boer K,Troost D,Jansen F,Nellist M,van den Ouweland AM,Geurts JJ,Spliet WG,Crino P,Aronica E

37-0400 was used in immunohistochemistry - paraffin section to report the neuropathological findings of a 32-year-old patient with a germ-line mutation in the TSC2 gene

Mon Dec 01 00:00:00 UTC 2008

IKK beta suppression of TSC1 links inflammation and tumor angiogenesis via the mTOR pathway.

Cell

Lee DF,Kuo HP,Chen CT,Hsu JM,Chou CK,Wei Y,Sun HL,Li LY,Ping B,Huang WC,He X,Hung JY,Lai CC,Ding Q,Su JL,Yang JY,Sahin AA,Hortobagyi GN,Tsai FJ,Tsai CH,Hung MC

37-0400 was used in western blot to investigate inflammation-mediated tumor angiogenesis

Fri Aug 10 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: Hamartin; HGNC:12362; MGC86987; OTTHUMP00000022439; OTTHUMP00000198378; Tuberous sclerosis 1 protein; tumor suppressor

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Gene Aliases: KIAA0243; LAM; TSC; TSC1

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UniProt ID: (Human) Q92574

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Entrez Gene ID: (Human) 7248

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Function(s)

protein binding GTPase regulator activity GTPase activating protein binding protein N-terminus binding chaperone binding

Process(es)

kidney development neural tube closure regulation of cell-matrix adhesion adaptive immune response rRNA export from nucleus regulation of translation potassium ion transport cell cycle arrest cell-matrix adhesion negative regulation of cell proliferation adult locomotory behavior negative regulation of macroautophagy negative regulation of translation hippocampus development cerebral cortex development cell projection organization negative regulation of TOR signaling response to insulin myelination memory T cell differentiation regulation of phosphoprotein phosphatase activity negative regulation of cell size regulation of protein kinase activity glucose import negative regulation of insulin receptor signaling pathway synapse organization protein stabilization protein heterooligomerization regulation of stress fiber assembly regulation of cell cycle positive regulation of focal adhesion assembly cardiac muscle cell differentiation activation of GTPase activity cellular response to oxygen-glucose deprivation regulation of neuron death

Performance Guarantee

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TSC1 Monoclonal Antibody (5C8A12)

TSC1 Antibody (37-0400) in IP

Product Details

37-0400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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