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TSC1 Antibody (TA802012) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC1 (RC213332, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC1. Positive lysates LY400132 (100 µg) and LC400132 (20 µg) can be purchased separately from OriGene.
Product Details
TA802012
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI2B2
Immunogen
Human recombinant protein fragment corresponding to amino acids 836-1164 of human TSC1 produced in E.coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hamartin; HGNC:12362; MGC86987; OTTHUMP00000022439; OTTHUMP00000198378; Tuberous sclerosis 1 protein; tumor suppressor
Gene Aliases: KIAA0243; LAM; TSC; TSC1
UniProt ID: (Human) Q92574
Entrez Gene ID: (Human) 7248
kidney development
neural tube closure
regulation of cell-matrix adhesion
adaptive immune response
rRNA export from nucleus
regulation of translation
potassium ion transport
cell cycle arrest
cell-matrix adhesion
negative regulation of cell proliferation
adult locomotory behavior
negative regulation of macroautophagy
negative regulation of translation
hippocampus development
cerebral cortex development
cell projection organization
negative regulation of TOR signaling
response to insulin
myelination
memory T cell differentiation
regulation of phosphoprotein phosphatase activity
negative regulation of cell size
regulation of protein kinase activity
glucose import
negative regulation of insulin receptor signaling pathway
synapse organization
protein stabilization
protein heterooligomerization
regulation of stress fiber assembly
regulation of cell cycle
positive regulation of focal adhesion assembly
cardiac muscle cell differentiation
activation of GTPase activity
cellular response to oxygen-glucose deprivation
regulation of neuron death
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