Invitrogen

TSC2 Monoclonal Antibody (3G9D9)

8 Published Figures

8 References

View all (52) TSC2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (8)

TSC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 10

TSC2 Antibody (37-0500) in IHC (P)

Immunohistochemistry analysis of Tuberin/TSC2 showing staining in the cytoplasm of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperatu... View More

TSC2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

TSC2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

37-0500

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 4 publications 4 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3G9D9

Immunogen

Recombinant human tuberin protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533293

Target Information

TSC2 (Tuberin, Tuberous sclerosis complex, TSC Complex Subunit 2), is implicated as a tumor suppressor and may function in vesicular transport, play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. TSC2 associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Phosphorylation of tuberin on Ser 939 and Thr 1462 regulates its interaction with hamartin, and is stimulated by various growth factors through the phosphoinositide 3-kinase/Akt pathway. TSC2 may have a function in vesicular transport, but may also play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. Interaction between TSC1 and TSC2 may facilitate vesicular docking. TSC2 specifically stimulates the intrinsic GTPase activity of the Ras related protein RAP1A and RAB5, suggesting a possible mechanism for its role in regulating cellular growth. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors and are involved in diseases such as lymphangioleiomyomatosis and TSC2 angiomyolipoma. Alternative splicing results in transcript variants of three different isoforms of TSC2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling.

Cell

Prentzell MT,Rehbein U,Cadena Sandoval M,De Meulemeester AS,Baumeister R,Brohée L,Berdel B,Bockwoldt M,Carroll B,Chowdhury SR,von Deimling A,Demetriades C,Figlia G,de Araujo MEG,Heberle AM,Heiland I,Holzwarth B,Huber LA,Jaworski J,Kedra M,Kern K,Kopach A,Korolchuk VI,van 't Land-Kuper I,Macias M,Nellist M,Palm W,Pusch S,Ramos Pittol JM,Reil M,Reintjes A,Reuter F,Sampson JR,Scheldeman C,Siekierska A,Stefan E,Teleman AA,Thomas LE,Torres-Quesada O,Trump S,West HD,de Witte P,Woltering S,Yordanov TE,Zmorzynska J,Opitz CA,Thedieck K

37-0500 was used in Immunoprecipitation to report that Ras GTPase-activating protein-binding proteins 1 and 2 reside at the cytoplasmic surface of lysosomes.

Thu Feb 04 00:00:00 UTC 2021

G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling.

Cell

37-0500 was used in Immunoprecipitation to report that Ras GTPase-activating protein-binding proteins 1 and 2 reside at the cytoplasmic surface of lysosomes.

Thu Feb 04 00:00:00 UTC 2021

The splicing factor U2AF1 contributes to cancer progression through a noncanonical role in translation regulation.

Genes & development

Palangat M,Anastasakis DG,Fei DL,Lindblad KE,Bradley R,Hourigan CS,Hafner M,Larson DR

37-0500 was used in Immunocytochemistry-immunoflourescence to show a noncanonical function of U2AF1 whereby it can directly bind mature mNA in the cytoplasm and negatively regulate mRNA translation, contributing to cancer progression.

Wed May 01 00:00:00 UTC 2019

Death-associated protein kinase 1 promotes growth of p53-mutant cancers.

The Journal of clinical investigation

Zhao J,Zhao D,Poage GM,Mazumdar A,Zhang Y,Hill JL,Hartman ZC,Savage MI,Mills GB,Brown PH

37-0500 was used in immunoprecipitation to identify molecular targets that suppress tumorigenesis in triple negative breast cancers.

Wed Jul 01 00:00:00 UTC 2015

Death-associated protein kinase 1 promotes growth of p53-mutant cancers.

The Journal of clinical investigation

Zhao J,Zhao D,Poage GM,Mazumdar A,Zhang Y,Hill JL,Hartman ZC,Savage MI,Mills GB,Brown PH

37-0500 was used in immunoprecipitation to identify molecular targets that suppress tumorigenesis in triple negative breast cancers.

Wed Jul 01 00:00:00 UTC 2015

Spatial control of the TSC complex integrates insulin and nutrient regulation of mTORC1 at the lysosome.

Cell

Menon S,Dibble CC,Talbott G,Hoxhaj G,Valvezan AJ,Takahashi H,Cantley LC,Manning BD

Published figure using TSC2 monoclonal antibody (Product # 37-0500) in Immunoprecipitation

Thu Feb 13 00:00:00 UTC 2014

TBC1D7 is a third subunit of the TSC1-TSC2 complex upstream of mTORC1.

Molecular cell

Dibble CC,Elis W,Menon S,Qin W,Klekota J,Asara JM,Finan PM,Kwiatkowski DJ,Murphy LO,Manning BD

Fri Aug 24 00:00:00 UTC 2012

TBC1D7 is a third subunit of the TSC1-TSC2 complex upstream of mTORC1.

Molecular cell

Dibble CC,Elis W,Menon S,Qin W,Klekota J,Asara JM,Finan PM,Kwiatkowski DJ,Murphy LO,Manning BD

Fri Aug 24 00:00:00 UTC 2012

Clinicopathological and immunohistochemical findings in an autopsy case of tuberous sclerosis complex.

Neuropathology : official journal of the Japanese Society of Neuropathology

Boer K,Troost D,Jansen F,Nellist M,van den Ouweland AM,Geurts JJ,Spliet WG,Crino P,Aronica E

37-0500 was used in immunohistochemistry - paraffin section to report the neuropathological findings of a 32-year-old patient with a germ-line mutation in the TSC2 gene

Mon Dec 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: OTTHUMP00000198394; protein phosphatase 1, regulatory subunit 160; Tuberin; Tuberous sclerosis 2 protein

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Gene Aliases: LAM; PPP1R160; TSC2; TSC4

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UniProt ID: (Human) P49815

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Entrez Gene ID: (Human) 7249

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Function(s)

GTPase activator activity protein binding phosphatase binding small GTPase binding protein homodimerization activity

Process(es)

neural tube closure negative regulation of protein kinase activity protein import into nucleus endocytosis cell cycle arrest heart development protein localization negative regulation of cell proliferation negative regulation of phosphatidylinositol 3-kinase signaling viral process vesicle-mediated transport regulation of endocytosis negative regulation of Wnt signaling pathway negative regulation of TOR signaling protein kinase B signaling positive regulation of GTPase activity regulation of insulin receptor signaling pathway negative regulation of insulin receptor signaling pathway insulin-like growth factor receptor signaling pathway positive chemotaxis regulation of small GTPase mediated signal transduction regulation of cell cycle negative regulation of protein kinase B signaling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TSC2 Monoclonal Antibody (3G9D9)

TSC2 Antibody (37-0500) in IHC (P)

Product Details

37-0500

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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