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Invitrogen
TSC2 Recombinant Rabbit Monoclonal Antibody (SC05-59)
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FIGURE: 1 / 7
TSC2 Antibody (MA5-32190) in ICC/IF
Immunofluorescence of TSC2 was performed using HAP1 wild-type and TSC2 KO cells that were transfected with a green or a far-red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) or methanol for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the TSC2 recombinant monoclon... View More
Product Details
MA5-32190
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SC05-59
Immunogen
Synthetic peptide within Human Tuberin aa 1760-1807
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809477
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
TSC2 (Tuberin, Tuberous sclerosis complex, TSC Complex Subunit 2), is implicated as a tumor suppressor and may function in vesicular transport, play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. TSC2 associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Phosphorylation of tuberin on Ser 939 and Thr 1462 regulates its interaction with hamartin, and is stimulated by various growth factors through the phosphoinositide 3-kinase/Akt pathway. TSC2 may have a function in vesicular transport, but may also play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. Interaction between TSC1 and TSC2 may facilitate vesicular docking. TSC2 specifically stimulates the intrinsic GTPase activity of the Ras related protein RAP1A and RAB5, suggesting a possible mechanism for its role in regulating cellular growth. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors and are involved in diseases such as lymphangioleiomyomatosis and TSC2 angiomyolipoma. Alternative splicing results in transcript variants of three different isoforms of TSC2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: OTTHUMP00000198394; protein phosphatase 1, regulatory subunit 160; renal carcinoma; Tsc2; Tuberin; tuberous sclerosis 2 homolog protein; Tuberous sclerosis 2 protein; Tuberous sclerosis 2 protein homolog
Gene Aliases: LAM; Nafld; PPP1R160; Rc; Tcs2; TSC2; TSC4
UniProt ID: (Human) P49815, (Rat) P49816
Entrez Gene ID: (Human) 7249, (Rat) 24855, (Mouse) 22084
neural tube closure
negative regulation of protein kinase activity
protein import into nucleus
endocytosis
cell cycle arrest
heart development
protein localization
negative regulation of cell proliferation
negative regulation of phosphatidylinositol 3-kinase signaling
viral process
vesicle-mediated transport
regulation of endocytosis
negative regulation of Wnt signaling pathway
negative regulation of TOR signaling
protein kinase B signaling
positive regulation of GTPase activity
regulation of insulin receptor signaling pathway
negative regulation of insulin receptor signaling pathway
insulin-like growth factor receptor signaling pathway
positive chemotaxis
regulation of small GTPase mediated signal transduction
regulation of cell cycle
negative regulation of protein kinase B signaling
response to hypoxia
acute-phase response
signal transduction
phosphatidylinositol 3-kinase signaling
cell projection organization
cellular response to insulin stimulus
negative regulation of MAP kinase activity
protein transport into plasma membrane raft
establishment of protein localization
negative regulation of cell size
positive regulation of transcription from RNA polymerase II promoter
glucose import
negative regulation of epithelial cell proliferation
protein homooligomerization
protein heterooligomerization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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