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FIGURE: 1 / 1
TSCOT Antibody (PA5-90509) in WB
Western blot analysis of TSCOT in extracts of various cell lines. Samples were incubated with TSCOT Polyclonal antibody (Product # PA5-90509) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-90509
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human SLC46A2 (NP_1490403)
Conjugate
Form
Liquid
Concentration
1.47 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806103
Product Specific Information
Immunogen sequence: MSPEVTCPRR GHLPRFHPRT WVEPVVASSQ VAASLYDAGL LLVVKASYGT GGSSNHSASP SPRGALEDQQ QRAISNFYII YNLVVGLSPL LSAYGLGWLS DRYHRKISIC; Positive Samples: LO2, Raji, MCF7, Mouse heart, Mouse brain, Mouse spleen; Cellular Location: Cell membrane, Multi-pass membrane protein
Target Information
The TSCOT gene, known as the Thymic Stromal Co-transporter, plays a crucial role in the gene expression regulation within thymic epithelial cells, essential for T cell development. It encodes a protein that bears weak homology to bacterial 12 transmembrane co-transporters. Research indicates that TSCOT is predominantly expressed in thymic tissues, although low levels of expression are detectable in other epithelial tissues such as skin and lungs. The gene has been co-analyzed with Foxn1 and Hoxa3, highlighting its significant role in organogenesis. Studies utilizing competitive reverse transcription-polymerase chain reaction (RT-PCR) have mapped TSCOT to specific genomic regions, verifying its expression in a tissue and cell-type specific manner. The introduction of a neoantigen into TSCOT-expressing cells demonstrates its capability to mediate antigen-specific T cell tolerance, offering potential applications in immunological therapies.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: putative thymic stromal co-transporter TSCOT; Solute carrier family 46 member 2; solute carrier family 46, member 2; thymic stromal co-transporter; Thymic stromal cotransporter homolog; thymic stromal cotransporter protein
Gene Aliases: 5430429N04Rik; Ly110; SLC46A2; TSCOT; TSO-1C12
UniProt ID: (Human) Q9BY10, (Mouse) Q8CA03
Entrez Gene ID: (Human) 57864, (Mouse) 30936
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