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Product Details
PA5-55513
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human TSEN2. Recombinant protein control fragment (Product #RP-94795).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2648988
Product Specific Information
Immunogen sequence: KTNMPIITSK RYQHSVEWAA ELMRRQGQDE STVRRILKDY TKPLEHPPVK RNEEAQVHDK LNSGMVSNME GTAGGERPSV VNGDSGKSGG VGDPREP
Highest antigen sequence identity to the following orthologs: Mouse - 45%, Rat - 48%.
Target Information
Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre- mRNA 3'-end processing factors, establishing a link between pre- tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HsSen2; tRNA-intron endonuclease Sen2; tRNA-splicing endonuclease subunit Sen2; TSEN2 tRNA splicing endonuclease subunit
Gene Aliases: PCH2B; SEN2; SEN2L; TSEN2
UniProt ID: (Human) G5E9Q3
Entrez Gene ID: (Human) 80746
Performance Guarantee
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