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Invitrogen
TSPO Polyclonal Antibody
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FIGURE: 1 / 5
TSPO Antibody (PA5-77884) in ICC/IF
Immunofluorescence analysis of TSPO was performed using THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TSPO Recombinant Rabbit Monoclonal Antibody (Product # PA5-77884) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27034, 1:2000... View More
Product Details
PA5-77884
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PBR. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.57 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2736329
Product Specific Information
Positive Control: HeLa, U87-MG, A431
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Mitochondrial peripheral-type benzodiazepine receptor (PBR) is an indispensable element of the steroidogenic machinery, where it mediates the delivery of cholesterol to the inner mitochondrial side chain cleavage cytochrome P-450 upon ligand activation. PBR is composed of three subunits, an isoquinoline binding site, a voltage-dependent anion channel and an adenine nucleotide carrier. PBR is genetically conserved from bacteria to humans and in humans is widely expressed in peripheral organs, whereas in the brain, it is sparse and located mainly in glial cells. Peroxisome proliferator perfluordecanoic acid (PFDA) inhibits the Leydig cell steroidogenesis by affecting PBR mRNA stability, thus inhibiting PBR expression, cholesterol transport into the mitochondria and subsequent steroid formation. A cytoplasmic protein, PRAX-1 ( peripheral benzodiazepine receptor-associated protein 1), is found to specifically interact with PBR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: benzodiazepine peripheral binding site; Mitochondrial benzodiazepine receptor; OTTHUMP00000028891; PBR; Peripheral-type benzodiazepine receptor; PKBS; Translocator protein; translocator protein (18kDa)
Gene Aliases: BPBS; BZRP; DBI; IBP; MBR; mDRC; PBR; PBS; pk18; PKBS; PTBR; TSPO
UniProt ID: (Human) P30536
Entrez Gene ID: (Human) 706
protein targeting to mitochondrion
steroid biosynthetic process
heme biosynthetic process
anion transport
chloride transport
lipid transport
apoptotic process
signal transduction
chemical synaptic transmission
aging
steroid metabolic process
cell proliferation
glial cell migration
response to manganese ion
response to vitamin B1
positive regulation of necrotic cell death
peripheral nervous system axon regeneration
adrenal gland development
negative regulation of protein ubiquitination
regulation of cholesterol transport
response to progesterone
negative regulation of tumor necrosis factor production
response to testosterone
response to drug
positive regulation of apoptotic process
negative regulation of nitric oxide biosynthetic process
behavioral response to pain
regulation of steroid biosynthetic process
positive regulation of mitochondrial depolarization
positive regulation of calcium ion transport
contact inhibition
positive regulation of glial cell proliferation
negative regulation of glial cell proliferation
cellular response to lipopolysaccharide
cellular response to zinc ion
cellular hypotonic response
maintenance of protein location in mitochondrion
negative regulation of mitophagy
negative regulation of ATP metabolic process
positive regulation of reactive oxygen species metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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