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TTI1 Antibody (A303-451A) in WB
Detection of human TTI1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TTI1 antibody (Product # A303-451A) used for WB at 0.04 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 6 µg/mg lysate. TTI1 was also immunoprecipitated by rabbit anti-TTI1 antibody A303-452A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and... View More
Product Details
A303-451A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1 and 50 of Human Tel2 Interacting Protein 1.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
Regulator of the DNA damage response (DDR). Part of the TTT complex that is required to stabilize protein levels of the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins. The TTT complex is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), ultraviolet (UV) and mitomycin C (MMC). Together with the TTT complex and HSP90 may participate in the proper folding of newly synthesized PIKKs. Promotes assembly, stabilizes and maintains the activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein SMG10; smg-10 homolog, nonsense mediated mRNA decay factor; Tel two interacting protein 1; TEL2-interacting protein 1 homolog; TELO2-interacting protein 1 homolog
Gene Aliases: KIAA0406; smg-10; SMG10; TTI1
UniProt ID: (Human) O43156
Entrez Gene ID: (Human) 9675
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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