Proteintech

TTL Polyclonal Antibody

TTL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TTL Antibody (13618-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 13618-1-AP (TTL antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

TTL Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

Product Details

13618-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 25 publications 25 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 6 publications 6 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Dog, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TTL Fusion Protein Ag4526 (1-377 aa encoded by BC036819)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MYTFVVRDE NSSVYAEVSR LLLATGHWKR LRRDNPRFNL MLGERNRLPF GRLGHEPGLV QLVNYYRGAD KLCRKASLVK LIKTSPELAE SCTWFPESYV IYPTNLKTPV APAQNGIQPP ISNSRTDERE FFLASYNRKK EDGEGNVWIA KSSAGAKGEG ILISSEASEL LDFIDNQGQV HVIQKYLEHP LLLEPGHRKF DIRSWVLVDH QYNIYLYREG VLRTASEPYH VDNFQDKTCH LTNHCIQKEY SKNYGKYEEG NEMFFKEFNQ YLTSALNITL ESSILLQIKH IIRNCLLSVE PAISTKHLPY QSFQLFGFDF MVDEELKVWL IEVNGAPACA QKLYAELCQG IVDIAISSVF PPPDVEQPQT QPAAFIKL (1-377 aa encoded by BC036819)

Target Information

Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chronic activation of tubulin tyrosination in HCM mice and human iPSC-engineered heart tissues improves heart function.

bioRxiv : the preprint server for biology

Pietsch N,Chen CY,Kupsch S,Bacmeister L,Geertz B,Herera-Rivero M,Voß H,Krämer E,Braren I,Westermann D,Schlüter H,Mearini G,Schlossarek S,van der Velden J,Caporizzo MA,Lindner D,Prosser BL,Carrier L

Wed Feb 07 00:00:00 UTC 2024

Altered Posttranslational Modification of Microtubules Contributes to Disturbed Enterocyte Morphology in Celiac Disease.

International journal of molecular sciences

Stricker S,Müller M,Zimmer KP,Jacob R

Mon Jan 30 00:00:00 UTC 2023

Altered Posttranslational Modification of Microtubules Contributes to Disturbed Enterocyte Morphology in Celiac Disease.

International journal of molecular sciences

Stricker S,Müller M,Zimmer KP,Jacob R

Mon Jan 30 00:00:00 UTC 2023

MFN2-dependent recruitment of ATAT1 coordinates mitochondria motility with alpha-tubulin acetylation and is disrupted in CMT2A.

bioRxiv : the preprint server for biology

Kumar A,Larrea D,Pero ME,Infante P,Conenna M,Shin GJ,Grueber WB,Di Marcotullio L,Area-Gomez E,Bartolini F

Thu Mar 16 00:00:00 UTC 2023

Mechanistic Analysis of CCP1 in Generating ΔC2 α-Tubulin in Mammalian Cells and Photoreceptor Neurons.

Biomolecules

Hotta T,Plemmons A,Gebbie M,Ziehm TA,Blasius TL,Johnson C,Verhey KJ,Pearring JN,Ohi R

Sun Feb 12 00:00:00 UTC 2023

Tubulin tyrosination regulates synaptic function and is disrupted in Alzheimer's disease.

Brain : a journal of neurology

Peris L,Parato J,Qu X,Soleilhac JM,Lanté F,Kumar A,Pero ME,Martínez-Hernández J,Corrao C,Falivelli G,Payet F,Gory-Fauré S,Bosc C,Blanca Ramirez M,Sproul A,Brocard J,Di Cara B,Delagrange P,Buisson A,Goldberg Y,Moutin MJ,Bartolini F,Andrieux A

Fri Jul 29 00:00:00 UTC 2022

Tubulin expression and modification in heart failure with preserved ejection fraction (HFpEF).

Scientific reports

Schulz L,Werner S,Böttner J,Adams V,Lurz P,Besler C,Thiele H,Büttner P

Wed Sep 21 00:00:00 UTC 2022

Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.

Journal of hazardous materials

Kang Y,Yin S,Liu J,Jiang Y,Huang Z,Chen L,Shao L

Tue Feb 15 00:00:00 UTC 2022

Crosstalk between acetylation and the tyrosination/detyrosination cycle of α-tubulin in Alzheimer's disease.

Frontiers in cell and developmental biology

Martínez-Hernández J,Parato J,Sharma A,Soleilhac JM,Qu X,Tein E,Sproul A,Andrieux A,Goldberg Y,Moutin MJ,Bartolini F,Peris L

Wed Sep 28 00:00:00 UTC 2022

Trans-cinnamaldehyde suppresses microtubule detyrosination and alleviates cardiac hypertrophy.

European journal of pharmacology

Tian J,Shan XL,Wang SN,Chen HH,Zhao P,Qian DD,Xu M,Guo W,Zhang C,Lu R

Wed Jan 05 00:00:00 UTC 2022

Posttranslational modification of microtubules by the MATCAP detyrosinase.

Science (New York, N.Y.)

Landskron L,Bak J,Adamopoulos A,Kaplani K,Moraiti M,van den Hengel LG,Song JY,Bleijerveld OB,Nieuwenhuis J,Heidebrecht T,Henneman L,Moutin MJ,Barisic M,Taraviras S,Perrakis A,Brummelkamp TR

Fri May 20 00:00:00 UTC 2022

RNA localization in confined cells depends on cellular mechanical activity and contributes to confined migration.

iScience

Moriarty RA,Mili S,Stroka KM

Fri Feb 18 00:00:00 UTC 2022

Microtubule-associated protein 1B dysregulates microtubule dynamics and neuronal mitochondrial transport in spinal muscular atrophy.

Human molecular genetics

Bora G,Hensel N,Rademacher S,Koyunoğlu D,Sunguroğlu M,Aksu-Mengeş E,Balcı-Hayta B,Claus P,Erdem-Yurter H

Thu Feb 25 00:00:00 UTC 2021

Pathogenic role of delta 2 tubulin in bortezomib-induced peripheral neuropathy.

Proceedings of the National Academy of Sciences of the United States of America

Pero ME,Meregalli C,Qu X,Shin GJ,Kumar A,Shorey M,Rolls MM,Tanji K,Brannagan TH,Alberti P,Fumagalli G,Monza L,Grueber WB,Cavaletti G,Bartolini F

Tue Jan 26 00:00:00 UTC 2021

TTL-Expression Modulates Epithelial Morphogenesis.

Frontiers in cell and developmental biology

Müller M,Ringer K,Hub F,Kamm N,Worzfeld T,Jacob R

Tue Feb 23 00:00:00 UTC 2021

MARK4 controls ischaemic heart failure through microtubule detyrosination.

Nature

Yu X,Chen X,Amrute-Nayak M,Allgeyer E,Zhao A,Chenoweth H,Clement M,Harrison J,Doreth C,Sirinakis G,Krieg T,Zhou H,Huang H,Tokuraku K,Johnston DS,Mallat Z,Li X

Tue Jun 01 00:00:00 UTC 2021

α-Tubulin detyrosination impairs mitotic error correction by suppressing MCAK centromeric activity.

The Journal of cell biology

Ferreira LT,Orr B,Rajendraprasad G,Pereira AJ,Lemos C,Lima JT,Guasch Boldú C,Ferreira JG,Barisic M,Maiato H

Mon Apr 06 00:00:00 UTC 2020

Depletion of Vasohibin 1 Speeds Contraction and Relaxation in Failing Human Cardiomyocytes.

Circulation research

Chen CY,Salomon AK,Caporizzo MA,Curry S,Kelly NA,Bedi K,Bogush AI,Krämer E,Schlossarek S,Janiak P,Moutin MJ,Carrier L,Margulies KB,Prosser BL

Fri Jul 03 00:00:00 UTC 2020

Tubulin detyrosination promotes human trophoblast syncytium formation.

Journal of molecular cell biology

Wang R,Yu R,Zhu C,Lin HY,Lu X,Wang H

Mon Dec 23 00:00:00 UTC 2019

Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure.

Nature medicine

Chen CY,Caporizzo MA,Bedi K,Vite A,Bogush AI,Robison P,Heffler JG,Salomon AK,Kelly NA,Babu A,Morley MP,Margulies KB,Prosser BL

Wed Aug 01 00:00:00 UTC 2018

Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure.

Nature medicine

Chen CY,Caporizzo MA,Bedi K,Vite A,Bogush AI,Robison P,Heffler JG,Salomon AK,Kelly NA,Babu A,Morley MP,Margulies KB,Prosser BL

Wed Aug 01 00:00:00 UTC 2018

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Nature

Liu S,Kwon M,Mannino M,Yang N,Renda F,Khodjakov A,Pellman D

Sat Sep 01 00:00:00 UTC 2018

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Nature

Liu S,Kwon M,Mannino M,Yang N,Renda F,Khodjakov A,Pellman D

Sat Sep 01 00:00:00 UTC 2018

Dissecting the role of the tubulin code in mitosis.

Methods in cell biology

Ferreira LT,Figueiredo AC,Orr B,Lopes D,Maiato H

Tue Dec 11 00:00:00 UTC 2018

PTEN suppresses axon outgrowth by down-regulating the level of detyrosinated microtubules.

PloS one

Kath C,Goni-Oliver P,Müller R,Schultz C,Haucke V,Eickholt B,Schmoranzer J

Fri Jul 13 00:00:00 UTC 2018

PTEN suppresses axon outgrowth by down-regulating the level of detyrosinated microtubules.

PloS one

Kath C,Goni-Oliver P,Müller R,Schultz C,Haucke V,Eickholt B,Schmoranzer J

Fri Jul 13 00:00:00 UTC 2018

Vasohibins encode tubulin detyrosinating activity.

Science (New York, N.Y.)

Nieuwenhuis J,Adamopoulos A,Bleijerveld OB,Mazouzi A,Stickel E,Celie P,Altelaar M,Knipscheer P,Perrakis A,Blomen VA,Brummelkamp TR

Fri Dec 15 00:00:00 UTC 2017

Vasohibins encode tubulin detyrosinating activity.

Science (New York, N.Y.)

Nieuwenhuis J,Adamopoulos A,Bleijerveld OB,Mazouzi A,Stickel E,Celie P,Altelaar M,Knipscheer P,Perrakis A,Blomen VA,Brummelkamp TR

Fri Dec 15 00:00:00 UTC 2017

The Effects of Guizhi Gancao Decoction on Pressure Overload-Induced Heart Failure and Posttranslational Modifications of Tubulin in Mice.

Evidence-based complementary and alternative medicine : eCAM

Chen HH,Zhao P,Tian J,Guo W,Xu M,Zhang C,Lu R

Thu Mar 17 00:00:00 UTC 2022

The Effects of Guizhi Gancao Decoction on Pressure Overload-Induced Heart Failure and Posttranslational Modifications of Tubulin in Mice.

Evidence-based complementary and alternative medicine : eCAM

Chen HH,Zhao P,Tian J,Guo W,Xu M,Zhang C,Lu R

Thu Mar 17 00:00:00 UTC 2022

Extracellular matrix stiffness and cell contractility control RNA localization to promote cell migration.

Nature communications

Wang T,Hamilla S,Cam M,Aranda-Espinoza H,Mili S

Thu Oct 12 00:00:00 UTC 2017

Extracellular matrix stiffness and cell contractility control RNA localization to promote cell migration.

Nature communications

Wang T,Hamilla S,Cam M,Aranda-Espinoza H,Mili S

Thu Oct 12 00:00:00 UTC 2017

Mitosis. Microtubule detyrosination guides chromosomes during mitosis.

Science (New York, N.Y.)

Barisic M,Silva e Sousa R,Tripathy SK,Magiera MM,Zaytsev AV,Pereira AL,Janke C,Grishchuk EL,Maiato H

Fri May 15 00:00:00 UTC 2015

Mitosis. Microtubule detyrosination guides chromosomes during mitosis.

Science (New York, N.Y.)

Barisic M,Silva e Sousa R,Tripathy SK,Magiera MM,Zaytsev AV,Pereira AL,Janke C,Grishchuk EL,Maiato H

Fri May 15 00:00:00 UTC 2015

AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation.

Nature communications

Herms A,Bosch M,Reddy BJ,Schieber NL,Fajardo A,Rupérez C,Fernández-Vidal A,Ferguson C,Rentero C,Tebar F,Enrich C,Parton RG,Gross SP,Pol A

Wed May 27 00:00:00 UTC 2015

AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation.

Nature communications

Herms A,Bosch M,Reddy BJ,Schieber NL,Fajardo A,Rupérez C,Fernández-Vidal A,Ferguson C,Rentero C,Tebar F,Enrich C,Parton RG,Gross SP,Pol A

Wed May 27 00:00:00 UTC 2015

Tubulin-tyrosine Ligase (TTL)-mediated Increase in Tyrosinated α-Tubulin in Injured Axons Is Required for Retrograde Injury Signaling and Axon Regeneration.

The Journal of biological chemistry

Song W,Cho Y,Watt D,Cavalli V

Fri Jun 05 00:00:00 UTC 2015

Tubulin-tyrosine Ligase (TTL)-mediated Increase in Tyrosinated α-Tubulin in Injured Axons Is Required for Retrograde Injury Signaling and Axon Regeneration.

The Journal of biological chemistry

Song W,Cho Y,Watt D,Cavalli V

Fri Jun 05 00:00:00 UTC 2015

Structural basis of tubulin tyrosination by tubulin tyrosine ligase.

The Journal of cell biology

Prota AE,Magiera MM,Kuijpers M,Bargsten K,Frey D,Wieser M,Jaussi R,Hoogenraad CC,Kammerer RA,Janke C,Steinmetz MO

Mon Feb 04 00:00:00 UTC 2013

Structural basis of tubulin tyrosination by tubulin tyrosine ligase.

The Journal of cell biology

Prota AE,Magiera MM,Kuijpers M,Bargsten K,Frey D,Wieser M,Jaussi R,Hoogenraad CC,Kammerer RA,Janke C,Steinmetz MO

Mon Feb 04 00:00:00 UTC 2013

Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells.

Journal of cell science

Zink S,Grosse L,Freikamp A,Bänfer S,Müksch F,Jacob R

Sat Dec 15 00:00:00 UTC 2012

Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells.

Journal of cell science

Zink S,Grosse L,Freikamp A,Bänfer S,Müksch F,Jacob R

Sat Dec 15 00:00:00 UTC 2012

Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells.

Journal of cell science

Zink S,Grosse L,Freikamp A,Bänfer S,Müksch F,Jacob R

Sat Dec 15 00:00:00 UTC 2012

Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells.

Journal of cell science

Zink S,Grosse L,Freikamp A,Bänfer S,Müksch F,Jacob R

Sat Dec 15 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: 2410003M22Rik; TTL; Tubulin--tyrosine ligase; tubulin-tyrosine ligase

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Gene Aliases: 2410003M22Rik; 2700049H19Rik; AI848570; TTL

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UniProt ID: (Human) Q8NG68, (Mouse) P38585, (Rat) Q9QXJ0

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Entrez Gene ID: (Human) 150465, (Mouse) 69737, (Rat) 171572

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Function(s)

tubulin-tyrosine ligase activity ATP binding nucleotide binding ligase activity

Process(es)

microtubule cytoskeleton organization cellular protein modification process regulation of axon extension

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TTL Polyclonal Antibody

TTL Antibody (13618-1-AP) in IHC (P)

Product Details

13618-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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