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Invitrogen
TTL Polyclonal Antibody
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FIGURE: 1 / 1
TTL Antibody (PA5-87576) in WB
Western blot analysis of TTL in extracts of various cell lines. Samples were incubated with TTL Polyclonal antibody (Product # PA5-87576) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-87576
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TTL (NP_7149231)
Conjugate
Form
Liquid
Concentration
1.44 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804264
Product Specific Information
Immunogen sequence: MYTFVVRDEN SSVYAEVSRL LLATGHWKRL RRDNPRFNLM LGERNRLPFG RLGHEPGLVQ LVNYYRGADK LCRKASLVKL IKTSPELAES CTWFPESYVI YPTNLKTPVA PAQNGIQPPI SNSRTDEREF FLASYNRKKE DGEGNVWIAK SSAGAKGEGI LISSEASELL DFIDNQGQVH VIQKYLEHPL LLEPGHRKFD IRSWVLVDHQ YNIYLYREGV LRTASEPYHV DNFQDKTCHL TNHCIQKEYS KNYGKYEEGN EMFFKEFNQY; Positive Samples: SH-SY5Y, HeLa, SKOV3, Mouse brain, Mouse lung, Mouse heart, Rat kidney
Target Information
Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2410003M22Rik; TTL; Tubulin--tyrosine ligase; tubulin-tyrosine ligase
Gene Aliases: 2410003M22Rik; 2700049H19Rik; AI848570; TTL
UniProt ID: (Human) Q8NG68, (Mouse) P38585, (Rat) Q9QXJ0
Entrez Gene ID: (Human) 150465, (Mouse) 69737, (Rat) 171572
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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