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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TWIST1 Antibodies

          Proteintech

          TWIST1 Polyclonal Antibody

          167 References
          View all (36) TWIST1 antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite TWIST1 Polyclonal Antibody

          • Antibody Testing Data (14)
          TWIST1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          TWIST1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 14

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          TWIST1 Antibody (25465-1-AP) in ICC/IF

          Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          TWIST1 Antibody in Immunocytochemistry (ICC/IF)
          TWIST1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TWIST1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TWIST1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TWIST1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TWIST1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TWIST1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TWIST1 Antibody in Western Blot (WB)
          TWIST1 Antibody in Western Blot (WB)
          TWIST1 Antibody in Western Blot (WB)
          TWIST1 Antibody in Western Blot (WB)
          TWIST1 Antibody in Western Blot (WB)
          TWIST1 Antibody in Western Blot (WB)
          TWIST1 Antibody in Immunoprecipitation (IP)

          Product Details

          25465-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 135 publications 135 publications

          Immunohistochemistry (IHC)

          -
          View 13 publications 13 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          View 7 publications 7 publications

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 3 publications 3 publications

          ChIP assay (ChIP)

          Assay-dependent
          View 4 publications 4 publications

          Miscellaneous PubMed (MISC)

          -
          View 2 publications 2 publications

          Not applicable (N/A)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          TWIST1-specific Fusion Protein Ag21924 (12-72 aa encoded by BC036704)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.38 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          The antibody reacts specifically with TWIST1 protein.

          Immunogen sequence: PADDSLSNS EEEPDRQQPP SGKRGGRKRR SSRRSAGGGA GPGGAAGGGV GGGDEPGSPA QG (12-72 aa encoded by BC036704)

          Target Information

          TWIST1 acts as a transcriptional regulator. It inhibits myogensis by sequestering E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. TWIST1 also represses proinflammatory cytokines such as TNFA and IL1B. TWIST also regulates cranial suture patterning and fusion and gene expression differentially depending on dimer composition. Mutations in the gene are implicated in Saethre-Chotzen syndrome, a disease characterized by coronal synostosis, brachycephaly, low front hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: B-HLH DNA binding protein; bHLHa38; Class A basic helix-loop-helix protein 38; H-twist; p ACS3; twist basic helix-loop-helix transcription factor 1; twist homolog 1; TWIST homolog of drosophila; Twist-related protein 1

          View more View less

          Gene Aliases: ACS3; BHLHA38; BPES2; BPES3; CRS; CRS1; CSO; SCS; TWIST; TWIST1

          View more View less

          UniProt ID: (Human) Q15672

          View more View less

          Entrez Gene ID: (Human) 7291

          View more View less

          Function(s)
          RNA polymerase II transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein domain specific binding protein homodimerization activity bHLH transcription factor binding protein heterodimerization activity E-box binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter ossification osteoblast differentiation in utero embryonic development neuron migration neural tube closure aortic valve morphogenesis mitral valve morphogenesis endocardial cushion morphogenesis cardiac neural crest cell migration involved in outflow tract morphogenesis transcription, DNA-templated muscle organ development positive regulation of gene expression positive regulation of epithelial to mesenchymal transition negative regulation of phosphatidylinositol 3-kinase signaling regulation of bone mineralization positive regulation of fatty acid beta-oxidation negative regulation of tumor necrosis factor production positive regulation of tumor necrosis factor production negative regulation of histone phosphorylation negative regulation of histone acetylation embryonic forelimb morphogenesis embryonic hindlimb morphogenesis negative regulation of peroxisome proliferator activated receptor signaling pathway outer ear morphogenesis odontogenesis embryonic digit morphogenesis negative regulation of apoptotic process negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of DNA damage response, signal transduction by p53 class mediator negative regulation of osteoblast differentiation positive regulation of angiogenesis negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process negative regulation of skeletal muscle tissue development embryonic cranial skeleton morphogenesis positive regulation of epithelial cell proliferation palate development cranial suture morphogenesis embryonic camera-type eye formation eyelid development in camera-type eye cellular response to growth factor stimulus cellular response to hypoxia positive regulation of monocyte chemotactic protein-1 production positive regulation of DNA-templated transcription, initiation positive regulation of cell motility negative regulation of oxidative phosphorylation uncoupler activity positive regulation of transcription regulatory region DNA binding negative regulation of cellular senescence positive regulation of interleukin-6 secretion negative regulation of double-strand break repair cell proliferation involved in heart valve development positive regulation of endocardial cushion to mesenchymal transition involved in heart valve formation
          It has to be done as per old AB suggested Products section.
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