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Invitrogen
TWIST2 Polyclonal Antibody
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FIGURE: 1 / 5
TWIST2 Antibody (PA5-99393) in ICC/IF
Immunofluorescent analysis of TWIST2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TWIST2 polyclonal antibody (Product # PA5-99393) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-99393
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TWIST2(Accession Q8WVJ9), corresponding to amino acid residues S10-S60.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2818326
Product Specific Information
Antibody detects endogenous levels of total Twist2.
Target Information
Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism. Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHa39; Class A basic helix-loop-helix protein 39; dermis expressed 1; Dermis-expressed protein 1; Dermo-1; dermo-1 protein; twist basic helix-loop-helix transcription factor 2; twist homolog 2; twist-related bHLH protein Dermo1; Twist-related protein 2
Gene Aliases: AMS; BBRSAY; BHLHA39; DERMO1; FFDD3; SETLSS; TWIST2
UniProt ID: (Human) Q8WVJ9, (Mouse) Q9D030, (Rat) P97831
Entrez Gene ID: (Human) 117581, (Mouse) 13345, (Rat) 59327
transcription, DNA-templated
multicellular organism development
cell differentiation
negative regulation of apoptotic process
negative regulation of osteoblast differentiation
negative regulation of transcription, DNA-templated
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
negative regulation of cell proliferation
positive regulation of keratinocyte proliferation
negative regulation of tumor necrosis factor production
negative regulation of DNA binding
negative regulation of molecular function
negative regulation of myeloid cell differentiation
embryonic cranial skeleton morphogenesis
face morphogenesis
cornea development in camera-type eye
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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