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Product Details
PA5-30677
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human TYW1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.92 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2548151
Product Specific Information
Recommended positive controls: A549, HeLa, HepG2, HCT116.
Predicted reactivity: Mouse (88%), Rat (89%), Zebrafish (84%), Xenopus laevis (87%), Bovine (93%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TYW1, also known as TYW1A, RSAFD1 or YPL207W, is a 732 amino acid protein containing one flavodoxin-like domain that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW1 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW1 binds to one 4Fe-4S cluster and is located on human chromosome 7. Defects in some of the genes on chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome, suggesting that TYW1 may play a role in these conditions.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Radical S-adenosyl methionine and flavodoxin domain-containing protein 1; radical S-adenosyl methionine and flavodoxin domains 1; S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1; tRNA wybutosine-synthesizing protein 1 homolog; tRNA-yW synthesizing protein 1 homolog A; tRNA-yW-synthesizing protein
Gene Aliases: RSAFD1; TYW1; TYW1A; YPL207W
UniProt ID: (Human) Q9NV66
Entrez Gene ID: (Human) 55253
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