Invitrogen

Talin Monoclonal Antibody (TA205)

Talin Antibody in Immunocytochemistry (ICC/IF)

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Talin Antibody (MA5-28133) in ICC/IF

Immunofluorescence analysis of Talin 1 was performed using MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Talin 1 Goat Polyclonal Antibody (Product # MA5-28133) at 5µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilution of 1:2000... View More

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Talin Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells lines tested owing to their inherent genetic constitution. Relative expression of Talin 1 was observed in MDA-MB-231 as compared to MCF7 which is reported to be low to negative for Talin 1 expression using Talin 1 Antibody (Product # MA5-28133) in western blot analysis. {RE}

Product Details

MA5-28133

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

5 µg/mL

Flow Cytometry (Flow)

1:25-1:100

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

TA205

Immunogen

Human platelet talin

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2745116

Product Specific Information

Mouse anti Human Talin antibody, clone TA205 recognizes human talin, a approximately 225 kDa cytoskeletal protein.

Target Information

Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The interaction of β-arrestin1 with talin1 driven by endothelin A receptor as a feature of α5β1 integrin activation in high-grade serous ovarian cancer.

Cell death & disease

Masi I,Ottavi F,Del Rio D,Caprara V,Vastarelli C,Giannitelli SM,Fianco G,Mozetic P,Buttarelli M,Ferrandina G,Scambia G,Gallo D,Rainer A,Bagnato A,Spadaro F,Rosanò L

MA5-28133 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot to highlight a new role of ETAR/ß-arr1 operating an inside-out integrin activation to modulate the metastatic process and suggest that in the new integrin-targeting programs might be considered that ETAR/ß-arr1 regulates Inta5ß1 functional pathway.

Mon Jan 30 00:00:00 UTC 2023

Bioinformatics

Protein Aliases: Talin-1; Talin-2

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Gene Aliases: ILWEQ; KIAA0320; KIAA1027; TLN; TLN1; TLN2

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UniProt ID: (Human) Q9Y490, (Human) Q9Y4G6

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Entrez Gene ID: (Human) 7094, (Human) 83660

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Function(s)

integrin binding structural constituent of cytoskeleton protein binding vinculin binding LIM domain binding actin filament binding cadherin binding involved in cell-cell adhesion actin binding structural molecule activity

Process(es)

cytoskeletal anchoring at plasma membrane cell-cell junction assembly cell adhesion platelet degranulation movement of cell or subcellular component muscle contraction cell-substrate junction assembly viral process cortical actin cytoskeleton organization IRE1-mediated unfolded protein response platelet aggregation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Talin Monoclonal Antibody (TA205)

Talin Antibody (MA5-28133) in ICC/IF

Product Details

MA5-28133

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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