Invitrogen

Tau Monoclonal Antibody (BT2)

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Tau Antibody (MN1010) in WB

Western blot was performed using Anti-Tau Monoclonal Antibody (BT2) (Product # MN1010) and 40-80 kDa bands corresponding to Tau were observed in Mouse and Rat Brain but not in Mouse Kidney or Mouse and Rat Lung tissues. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Kidney (Lane 3), Mouse Lung (Lane 4) or Rat Lung (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2... View More

Tau Antibody in Immunocytochemistry (ICC/IF)

Tau Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Tau Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across Mouse Brain, Rat Brain, Mouse Kidney, Mouse Lung, Rat Lung owing to their inherent genetic constitution. Relative expression of Tau was observed in Mouse and Rat Brain as compared to Kidney and Lung tissues using Anti-Tau Monoclonal Antibody (BT2) (Product # MN1010) in Western Blot. {RE}

Product Details

MN1010

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

ELISA (ELISA)

2-10 µg/mL

View 13 publications 13 publications

Mass Spectrometry (MS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

BT2

Immunogen

Purified bovine Tau (aa 194-198).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice (domestic); Dry ice (international)

AB_10975238

Product Specific Information

MN1010 recognizes normal Tau from human, rat, monkey, and bovine brain. No cross reactivity with PHF-Tau has been observed. The epitope of this antibody has been mapped on human Tau between residue 194 and 198 (numbering according to human Tau40), corresponding to the amnio acid sequence RSGYS.

MN1010 detects Tau which has a predicted molecular weight of approximately 79 kDa.

This product is a Low Endotoxin formulation.

Purity is >95% as determined by SDS-PAGE.

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Immunotherapy with Cleavage-Specific 12A12mAb Reduces the Tau Cleavage in Visual Cortex and Improves Visuo-Spatial Recognition Memory in Tg2576 AD Mouse Model.

Pharmaceutics

Latina V,De Introna M,Caligiuri C,Loviglio A,Florio R,La Regina F,Pignataro A,Ammassari-Teule M,Calissano P,Amadoro G

MN1010 was used in Western Blotting to find out that non-invasive administration of 12 A12mAb markedly reduced the pathological accumulation of both truncated tau and Aβ in the V1 area, correlated to significant improvement in visual recognition memory performance along with local increase in two direct readouts of cortical synaptic plasticity, including the dendritic spine density and the expression level of activity-regulated cytoskeleton protein Arc/Arg3.1.

Fri Feb 03 00:00:00 UTC 2023

Immunotherapy with Cleavage-Specific 12A12mAb Reduces the Tau Cleavage in Visual Cortex and Improves Visuo-Spatial Recognition Memory in Tg2576 AD Mouse Model.

Pharmaceutics

Latina V,De Introna M,Caligiuri C,Loviglio A,Florio R,La Regina F,Pignataro A,Ammassari-Teule M,Calissano P,Amadoro G

Fri Feb 03 00:00:00 UTC 2023

Mass spectrometric simultaneous quantification of tau species in plasma shows differential associations with amyloid and tau pathologies.

Nature aging

Montoliu-Gaya L,Benedet AL,Tissot C,Vrillon A,Ashton NJ,Brum WS,Lantero-Rodriguez J,Stevenson J,Nilsson J,Sauer M,Rahmouni N,Brinkmalm G,Lussier FZ,Pascoal TA,Skoog I,Kern S,Zetterberg H,Paquet C,Gobom J,Rosa-Neto P,Blennow K

MN1010 was used in Mass Spectrometry to support the differential association of blood p-tau variants with AD pathology, and our method offers a potential tool for disease staging in clinical trials.

Thu Jun 01 00:00:00 UTC 2023

TFEB regulates lysosomal exocytosis of tau and its loss of function exacerbates tau pathology and spreading.

Molecular psychiatry

Xu Y,Du S,Marsh JA,Horie K,Sato C,Ballabio A,Karch CM,Holtzman DM,Zheng H

Published figure using Tau monoclonal antibody (Product # MN1010) in ELISA

Fri Oct 01 00:00:00 UTC 2021

Systemic delivery of a specific antibody targeting the pathological N-terminal truncated tau peptide reduces retinal degeneration in a mouse model of Alzheimer's Disease.

Acta neuropathologica communications

Latina V,Giacovazzo G,Cordella F,Balzamino BO,Micera A,Varano M,Marchetti C,Malerba F,Florio R,Ercole BB,La Regina F,Atlante A,Coccurello R,Di Angelantonio S,Calissano P,Amadoro G

MN1010 was used in Western Blotting to find that 12A12mAb administration also exerts a beneficial action on biochemical, morphological and metabolic parameters (i.e. APP/Aβ processing, tau hyperphosphorylation, neuroinflammation, synaptic proteins, microtubule stability, mitochondria-based energy production, neuronal death) associated with ocular injury in the AD phenotype.

Tue Mar 09 00:00:00 UTC 2021

Systemic delivery of a specific antibody targeting the pathological N-terminal truncated tau peptide reduces retinal degeneration in a mouse model of Alzheimer's Disease.

Acta neuropathologica communications

Latina V,Giacovazzo G,Cordella F,Balzamino BO,Micera A,Varano M,Marchetti C,Malerba F,Florio R,Ercole BB,La Regina F,Atlante A,Coccurello R,Di Angelantonio S,Calissano P,Amadoro G

Tue Mar 09 00:00:00 UTC 2021

Genome-wide CRISPR screen identifies protein pathways modulating tau protein levels in neurons.

Communications biology

Sanchez CG,Acker CM,Gray A,Varadarajan M,Song C,Cochran NR,Paula S,Lindeman A,An S,McAllister G,Alford J,Reece-Hoyes J,Russ C,Craig L,Capre K,Doherty C,Hoffman GR,Luchansky SJ,Polydoro M,Dolmetsch R,Elwood F

MN1010 was used in Immunocytochemistry to identify modulators of endogenous tau protein for the first time through a a genome-wide CRISPR screen.

Mon Jun 14 00:00:00 UTC 2021

Genome-wide CRISPR screen identifies protein pathways modulating tau protein levels in neurons.

Communications biology

MN1010 was used in Immunocytochemistry to identify modulators of endogenous tau protein for the first time through a a genome-wide CRISPR screen.

Mon Jun 14 00:00:00 UTC 2021

Development and Validation of a High Sensitivity Assay for Measuring p217 + tau in Cerebrospinal Fluid.

Journal of Alzheimer's disease : JAD

Triana-Baltzer G,Van Kolen K,Theunis C,Moughadam S,Slemmon R,Mercken M,Galpern W,Sun H,Kolb H

MN1010 was used in an ELISA assay to describe the development and validation of a novel sELISA for measuring CSF tau species containing phosphorylation at threonines 212 & 217, aka p217 + tau, using the PT3 antibody.

Fri Sep 10 00:00:00 UTC 2021

Development and Validation of a High Sensitivity Assay for Measuring p217 + tau in Cerebrospinal Fluid.

Journal of Alzheimer's disease : JAD

Triana-Baltzer G,Van Kolen K,Theunis C,Moughadam S,Slemmon R,Mercken M,Galpern W,Sun H,Kolb H

Fri Sep 10 00:00:00 UTC 2021

Chronic PD-1 Checkpoint Blockade Does Not Affect Cognition or Promote Tau Clearance in a Tauopathy Mouse Model.

Frontiers in aging neuroscience

Lin Y,Rajamohamedsait HB,Sandusky-Beltran LA,Gamallo-Lana B,Mar A,Sigurdsson EM

Published figure using Tau monoclonal antibody (Product # MN1010) in ELISA

Mon Sep 28 00:00:00 UTC 2020

A post-translational modification signature defines changes in soluble tau correlating with oligomerization in early stage Alzheimer's disease brain.

Acta neuropathologica communications

Ercan-Herbst E,Ehrig J,Schöndorf DC,Behrendt A,Klaus B,Gomez Ramos B,Prat Oriol N,Weber C,Ehrnhoefer DE

Published figure using Tau monoclonal antibody (Product # MN1010) in ELISA

Tue Dec 03 00:00:00 UTC 2019

Highly sensitive quantification of Alzheimer's disease biomarkers by aptamer-assisted amplification.

Theranostics

Chan HN,Xu D,Ho SL,He D,Wong MS,Li HW

MN1010 was used in Western Blotting to show how the developed assay holds tremendous clinical potential for early diagnosis of AD and monitoring of its progression.

Thu Jun 18 00:00:00 UTC 2020

Highly sensitive quantification of Alzheimer's disease biomarkers by aptamer-assisted amplification.

Theranostics

Chan HN,Xu D,Ho SL,He D,Wong MS,Li HW

MN1010 was used in Western Blotting to show how the developed assay holds tremendous clinical potential for early diagnosis of AD and monitoring of its progression.

Thu Jun 18 00:00:00 UTC 2020

Probing Conformational Dynamics of Tau Protein by Hydrogen/Deuterium Exchange Mass Spectrometry.

Journal of the American Society for Mass Spectrometry

Huang RY,Iacob RE,Sankaranarayanan S,Yang L,Ahlijanian M,Tao L,Tymiak AA,Chen G

MN1010 was used in an ELISA assay to illustrate the practical utility of orthogonal analytical methodologies for the characterization of protein higher order structure.

Mon Jan 01 00:00:00 UTC 2018

Detection of Aggregation-Competent Tau in Neuron-Derived Extracellular Vesicles.

International journal of molecular sciences

Guix FX,Corbett GT,Cha DJ,Mustapic M,Liu W,Mengel D,Chen Z,Aikawa E,Young-Pearse T,Kapogiannis D,Selkoe DJ,Walsh DM

Published figure using Tau monoclonal antibody (Product # MN1010) in ELISA

Tue Feb 27 00:00:00 UTC 2018

Detection of Aggregation-Competent Tau in Neuron-Derived Extracellular Vesicles.

International journal of molecular sciences

Guix FX,Corbett GT,Cha DJ,Mustapic M,Liu W,Mengel D,Chen Z,Aikawa E,Young-Pearse T,Kapogiannis D,Selkoe DJ,Walsh DM

Published figure using Tau monoclonal antibody (Product # MN1010) in ELISA

Tue Feb 27 00:00:00 UTC 2018

Variable tau accumulation in murine models with abnormal prion protein deposits.

Journal of the neurological sciences

Piccardo P,King D,Brown D,Barron RM

MN1010 was used in Immunohistochemistry on paraffin embedded tissues-immunofluorescence to examine variable tau accumulation in murine models with abnormal prion protein deposits.

Fri Dec 15 00:00:00 UTC 2017

Variable tau accumulation in murine models with abnormal prion protein deposits.

Journal of the neurological sciences

Piccardo P,King D,Brown D,Barron RM

MN1010 was used in Immunohistochemistry on paraffin embedded tissues-immunofluorescence to examine variable tau accumulation in murine models with abnormal prion protein deposits.

Fri Dec 15 00:00:00 UTC 2017

A validated antibody panel for the characterization of tau post-translational modifications.

Molecular neurodegeneration

Ercan E,Eid S,Weber C,Kowalski A,Bichmann M,Behrendt A,Matthes F,Krauss S,Reinhardt P,Fulle S,Ehrnhoefer DE

Published figure using Tau monoclonal antibody (Product # MN1010) in ELISA

Tue Nov 21 00:00:00 UTC 2017

Membrane association and release of wild-type and pathological tau from organotypic brain slice cultures.

Cell death & disease

Croft CL,Wade MA,Kurbatskaya K,Mastrandreas P,Hughes MM,Phillips EC,Pooler AM,Perkinton MS,Hanger DP,Noble W

MN1010 was used in ELISA to examine tau distribution and release using organotypic brain slice cultures

Thu Mar 16 00:00:00 UTC 2017

Passive immunization with phospho-tau antibodies reduces tau pathology and functional deficits in two distinct mouse tauopathy models.

PloS one

Sankaranarayanan S,Barten DM,Vana L,Devidze N,Yang L,Cadelina G,Hoque N,DeCarr L,Keenan S,Lin A,Cao Y,Snyder B,Zhang B,Nitla M,Hirschfeld G,Barrezueta N,Polson C,Wes P,Rangan VS,Cacace A,Albright CF,Meredith J,Trojanowski JQ,Lee VM,Brunden KR,Ahlijanian M

MN1010 was used in an ELISA assay to provide evidence supporting the use of tau immunotherapy as a potential treatment option for AD and other tauopathies.

Thu Feb 04 00:00:00 UTC 2016

Analysis of tau post-translational modifications in rTg4510 mice, a model of tau pathology.

Molecular neurodegeneration

Song L,Lu SX,Ouyang X,Melchor J,Lee J,Terracina G,Wang X,Hyde L,Hess JF,Parker EM,Zhang L

MN1010 was used in ELISA to use rTg4510 mice to elucidate pathogenesis of tau-induced disease.

Thu Mar 26 00:00:00 UTC 2015

Insulin therapy modulates mitochondrial dynamics and biogenesis, autophagy and tau protein phosphorylation in the brain of type 1 diabetic rats.

Biochimica et biophysica acta

Santos RX,Correia SC,Alves MG,Oliveira PF,Cardoso S,Carvalho C,Duarte AI,Santos MS,Moreira PI

MN1010 was used in western blot to study mitochondrial function, autophagy and phosphorylated tau in type 1 diabetic rat cerebral cortex and the effects of insulin treatment

Tue Jul 01 00:00:00 UTC 2014

Characterization of novel CSF Tau and ptau biomarkers for Alzheimer's disease.

PloS one

Meredith JE,Sankaranarayanan S,Guss V,Lanzetti AJ,Berisha F,Neely RJ,Slemmon JR,Portelius E,Zetterberg H,Blennow K,Soares H,Ahlijanian M,Albright CF

MN1010 was used in an ELISA assay to provide novel tools to investigate CSF tau and ptau as biomarkers for other neurodegenerative diseases.

Sat Aug 02 00:00:00 UTC 2014

Characterization of novel CSF Tau and ptau biomarkers for Alzheimer's disease.

PloS one

Meredith JE,Sankaranarayanan S,Guss V,Lanzetti AJ,Berisha F,Neely RJ,Slemmon JR,Portelius E,Zetterberg H,Blennow K,Soares H,Ahlijanian M,Albright CF

MN1010 was used in an ELISA assay to provide novel tools to investigate CSF tau and ptau as biomarkers for other neurodegenerative diseases.

Sat Aug 02 00:00:00 UTC 2014

Early postnatal lead exposure induces tau phosphorylation in the brain of young rats.

Acta biologica Hungarica

Rahman A,Khan KM,Al-Khaledi G,Khan I,Attur S

MN1010 was used in western blot to study tau phosphorylation in response to early post-natal exposure to lead

Sat Dec 01 00:00:00 UTC 2012

Decrease in brain soluble amyloid precursor protein β (sAPPβ) in Alzheimer's disease cortex.

Journal of neuroscience research

Wu G,Sankaranarayanan S,Hsieh SH,Simon AJ,Savage MJ

MN1010 was used in ELISA to study the sAPPb decrease in Alzheimer disease cortex

Wed Jun 01 00:00:00 UTC 2011

Long term changes in phospho-APP and tau aggregation in the 3xTg-AD mice following cerebral ischemia.

Neuroscience letters

Koike MA,Garcia FG,Kitazawa M,Green KN,Laferla FM

MN1010 was used in western blot to investigate the phosphorylation and aggregation of tau protein in the 3xTg-AD mice

Mon May 09 00:00:00 UTC 2011

Detailed immunohistochemical characterization of temporal and spatial progression of Alzheimer's disease-related pathologies in male triple-transgenic mice.

BMC neuroscience

Mastrangelo MA,Bowers WJ

MN1010 was used in immunohistochemistry to study the temporal and spatial progression of AD-like pathology in male 3xTg-AD mice

Tue Aug 12 00:00:00 UTC 2008

Suppression of Parkin enhances nigrostriatal and motor neuron lesion in mice over-expressing human-mutated tau protein.

Human molecular genetics

Menéndez J,Rodríguez-Navarro JA,Solano RM,Casarejos MJ,Rodal I,Guerrero R,Sánchez MP,Avila J,Mena MA,de Yébenes JG

MN1010 was used in immunohistochemistry to investigate the role of Parkin in nigrostriatal and motor neuron integrity in mice

Sat Jul 01 00:00:00 UTC 2006

Assembly of alpha4beta2 nicotinic acetylcholine receptors assessed with functional fluorescently labeled subunits: effects of localization, trafficking, and nicotine-induced upregulation in clonal mammalian cells and in cultured midbrain neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Nashmi R,Dickinson ME,McKinney S,Jareb M,Labarca C,Fraser SE,Lester HA

MN1010 was used in immunohistochemistry to study alpha4beta2 nicotinic acetylcholine receptor assembly

Wed Dec 17 00:00:00 UTC 2003

The Journal of neuroscience : the official journal of the Society for Neuroscience

Nashmi R,Dickinson ME,McKinney S,Jareb M,Labarca C,Fraser SE,Lester HA

MN1010 was used in immunohistochemistry to study alpha4beta2 nicotinic acetylcholine receptor assembly

Wed Dec 17 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: FLJ31424; FTDP17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; MGC138549; microtubule associated protein tau; Microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; Paired helical filament-tau; PHF-tau; PHFtau; protein phosphatase 1, regulatory subunit 103; Tau microtubule-associated protein; Tau-4; Tau5

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Gene Aliases: BOS_19251; DDPAC; FTDP-17; MAPT; MAPTL; MSTD; Mtapt; MTBT1; MTBT2; PPND; PPP1R103; pTau; RNPTAU; TAU

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UniProt ID: (Bovine) P29172, (Human) P10636

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Entrez Gene ID: (Bovine) 281296, (Human) 4137, (Rat) 29477

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Function(s)

protein binding microtubule binding SH3 domain binding protein kinase binding apolipoprotein binding lipoprotein particle binding structural constituent of cytoskeleton enzyme binding protein complex binding protein phosphatase 2A binding protein domain specific binding

Process(es)

microtubule cytoskeleton organization neuron migration adult walking behavior axo-dendritic transport regulation of autophagy positive regulation of microtubule polymerization negative regulation of intracellular transport positive regulation of axon extension mitochondrion transport along microtubule axon extension regulation of microtubule-based movement brain development female pregnancy response to nutrient intrinsic apoptotic signaling pathway in response to oxidative stress response to organic substance regulation of microtubule polymerization generation of neurons apoptotic process microtubule-based process axonogenesis neuron differentiation

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Tau Monoclonal Antibody (BT2)

Tau Antibody (MN1010) in WB

Product Details

MN1010

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Mass Spectrometry (MS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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