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FIGURE: 1 / 1
TdT Antibody (PA5-92852) in WB
Western blot analysis of TdT in extracts of various cell lines. Samples were incubated with TdT Polyclonal antibody (Product # PA5-92852) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-92852
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 260-509 of human TdT (NP_0040793)
Conjugate
Form
Liquid
Concentration
0.37 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806515
Product Specific Information
Positive Samples: Mouse liver, Mouse thymus, Rat liver; Cellular Location: Nucleus
Immunogen sequence: LKTSEKWFRM GFRTLSKVRS DKSLKFTRMQ KAGFLYYEDL VSCVTRAEAE AVSVLVKEAV WAFLPDAFVT MTGGFRRGKK MGHDVDFLIT SPGSTEDEEQ LLQKVMNLWE KKGLLLYYDL VESTFEKLRL PSRKVDALDH FQKCFLIFKL PRQRVDSDQS SWQEGKTWKA IRVDLVLCPY ERRAFALLGW TGSRQFERDL RRYATHERKM ILDNHALYDK TKRIFLKAES EEEIFAHLGL DYIEPWERNA
Target Information
Terminal Deoxynucleotidyl Transferase (TdT) is a DNA polymerase located in the cell nucleus which catalyses the polymerization of deoxynucleotides at the 3' hydroxyl ends of oligo or polydeoxynucleotide initiators and functions without a template. TdT is considered to be a highly specific marker for the diagnosis and classification of acute lymphoblastic lymphoma/leuksemias. The determination of TdT expression is most valuable when it is different to differentiate histologically between lymphoblastic lymphoma and Burkitt's lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deoxynucleotidyltransferase terminal; deoxynucleotidyltransferase, terminal; DNA nucleotidylexotransferase; Nucleosidetriphosphate DNA Deoxynucleotidylexotransferase; nucleosidetriphosphate:DNA deoxynucleotidylexotransferase; TDT; Terminal addition enzyme; terminal deoxynucleotidyl transferase; Terminal deoxynucleotidyltransferase; terminal deoxyribonucleotidyltransferase; Terminal transferase
Gene Aliases: DNTT; TDT
UniProt ID: (Human) P04053, (Mouse) P09838
Entrez Gene ID: (Human) 1791, (Mouse) 21673, (Rat) 294051
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