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Invitrogen
TdT Monoclonal Antibody (19-3), APC, eBioscience™
FIGURE: 1 / 1
TdT Antibody (17-5846-82) in Flow
Staining of C57BL/6 thymocytes with CD4 Monoclonal Antibody, eFluor® 450 (Product # 48-0042-82) and CD8 Monoclonal Antibody, FITC (Product # 11-0081-82) followed by fixation and permeabilization with the Foxp3 Stainng Buffers (Product # 00-5523-00) and subsequent staining with 0.5 µg of TdT Monoclonal Antibody, APC. Mouse IgG2b kappa Isotype Control, APC (Product # 17-4031-82) is shown in grey, CD4 single positives in blue, CD8 single positives in purple, and double positives in orange.
Product Details
17-5846-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
19-3
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2762816
Product Specific Information
Description: This 19-3 monoclonal antibody recognizes human and mouse terminal-deoxynucleotidyl transferase (TdT), a 60 kDa polymerase responsible for the template-independent addition of N-nucleotides at gene segment junctions of developing lymphocytes. Expression is found in the nucleus of immature lymphocytes but not in mature lymphocytes or non-lymphoid cells. Tdt plays a critical role during TCR and Ig gene rearrangement. Regulation of expression in the mouse thymus correlates with T cell selection; decreased or absent after positive selection. Additionally Tdt has been shown to be present in lymphoma or lymphoblastic leukemia cells.
Applications Reported: This 19-3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 19-3 antibody has been tested by intracellular staining using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5521-00) followed by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Target Information
Terminal Deoxynucleotidyl Transferase (TdT) is a DNA polymerase located in the cell nucleus which catalyses the polymerization of deoxynucleotides at the 3' hydroxyl ends of oligo or polydeoxynucleotide initiators and functions without a template. TdT is considered to be a highly specific marker for the diagnosis and classification of acute lymphoblastic lymphoma/leuksemias. The determination of TdT expression is most valuable when it is different to differentiate histologically between lymphoblastic lymphoma and Burkitt's lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deoxynucleotidyltransferase terminal; deoxynucleotidyltransferase, terminal; DNA nucleotidylexotransferase; Nucleosidetriphosphate DNA Deoxynucleotidylexotransferase; nucleosidetriphosphate:DNA deoxynucleotidylexotransferase; TDT; Terminal addition enzyme; terminal deoxynucleotidyl transferase; Terminal deoxynucleotidyltransferase; terminal deoxyribonucleotidyltransferase; Terminal transferase
Gene Aliases: DNTT; TDT
UniProt ID: (Human) P04053, (Mouse) P09838
Entrez Gene ID: (Human) 1791, (Mouse) 21673
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