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Themis Antibody (MA5-24337) in WB
Western blot analysis of Themis in mouse thymus tissue. Samples were incubated in Themis monoclonal antibody (Product # MA5-24337) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Rat IgG secondary antibody. A specific band was detected for Themis at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
MA5-24337
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
719945
Immunogen
E. coli-derived recombinant mouse Themis Lys122-Lys237
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2606930
Product Specific Information
In Western blots, 100% cross-reactivity with recombinant human (rh) Themis (aa 2-282) is observed under non-reducing conditions, and approximately 10% cross-reactivity with rhThemis (aa 2-282) is observed under reducing conditions.
Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
Target Information
Themis is a cytoplasmic protein that is essential for positive selection and the development of single-positive thymocytes. Highest levels of expression are found in CD4+CD8+ double-positive thymocytes, with lower expression found in mature T cells. In addition to Grb1, Themis has been found to associate with PLC gamma 1 and LAT and is quickly phosphorylated by SLP-76 following TCR signaling. There are 5 isoforms of mouse Themis that are produced as a result of alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bA325O24.3; bA325O24.4; FLJ40584; Gasp; Grb2-associating protein; MGC163388; Protein THEMIS; Protein thylex; Thymocyte-expressed molecule involved in selection
Gene Aliases: E430004N04Rik; Gasp; Spot; Themis; thylex; Tsepa
UniProt ID: (Mouse) Q8BGW0
Entrez Gene ID: (Mouse) 210757
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