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Invitrogen
Themis Recombinant Rabbit Monoclonal Antibody (JE55-73)
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FIGURE: 1 / 6
Themis Antibody (MA5-42501) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of Themis in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Themis Recombinant Monoclonal Antibody (Product # MA5-42501) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue)... View More
Product Details
MA5-42501
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE55-73
Immunogen
Synthetic peptide within C-terminal Human Themis
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911642
Product Specific Information
Positive Control: Jurkat cell lysates, 293T, rat spleen tissue, human appendix tissue, human spleen tissue, Jurkat.
Subcellular Location: Nucleus, Cytoplasm.
Target Information
Themis is a cytoplasmic protein that is essential for positive selection and the development of single-positive thymocytes. Highest levels of expression are found in CD4+CD8+ double-positive thymocytes, with lower expression found in mature T cells. In addition to Grb1, Themis has been found to associate with PLC gamma 1 and LAT and is quickly phosphorylated by SLP-76 following TCR signaling. There are 5 isoforms of mouse Themis that are produced as a result of alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bA325O24.3; bA325O24.4; FLJ40584; GRB2-associated protein; MGC163388; Protein THEMIS; signaling phosphoprotein specific for T cells; thymocyte expressed molecule involved in selection; thymocyte selection associated; thymocyte selection pathway associated; Thymocyte-expressed molecule involved in selection
Gene Aliases: C6orf190; C6orf207; GASP; RGD1560849; SPOT; THEMIS; TSEPA
UniProt ID: (Human) Q8N1K5
Entrez Gene ID: (Human) 387357, (Rat) 498985
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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