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Invitrogen
Thioredoxin 1 Polyclonal Antibody
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FIGURE: 1 / 9
Thioredoxin 1 Antibody (PA5-95589) in ICC/IF
Immunocytochemistry analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody (Product # PA5-95589) . Thioredoxin TRX was detected in a section of NIH3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Thioredoxin TRX antibody (Product # PA5-95589) overnight at 4°... View More
Product Details
PA5-95589
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived mouse Thioredoxin TRX recombinant protein (Position: V2-A105).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807391
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat kidney tissue, rat small intestine tissue, rat spleen tissue, rat lung tissue, rat C6 whole cell, mouse kidney tissue, mouse small intestine tissue, mouse lung tissue, mouse heart tissue, mouse NIH/3T3 whole cell. IHC: mouse small intestine tissue, rat spleen tissue. ICC/IF: NIH3T3 cell, LLC Cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide-isomerase (PDI) and a number of transcription factors such as p53, NF-kB and AP-1 (T1-151). Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADF; ATL-derived factor; DKFZp686B1993; MGC61975; RP11-427L11.1; Thioredoxin; Trx
Gene Aliases: ADF; AW550880; Trx1; Txn; Txn1
UniProt ID: (Rat) P11232, (Mouse) P10639
Entrez Gene ID: (Rat) 116484, (Mouse) 22166
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
glycerol ether metabolic process
response to radiation
response to selenium ion
response to activity
positive regulation of peptidyl-serine phosphorylation
regulation of protein import into nucleus, translocation
cellular response to drug
response to drug
positive regulation of DNA binding
cell redox homeostasis
negative regulation of protein export from nucleus
response to axon injury
oxidation-reduction process
cellular response to antibiotic
cellular response to glucose stimulus
cellular response to hyperoxia
response to dexamethasone
response to thyroxine
negative regulation of hydrogen peroxide-induced cell death
sulfate assimilation
protein folding
transport
cellular response to oxidative stress
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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