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Thymidylate synthase Polyclonal Antibody

Thymidylate synthase Antibody in Immunocytochemistry (ICC/IF)

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Thymidylate synthase Antibody (15047-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using (Product # 15047-1-AP) (Thymidylate synthase antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Thymidylate synthase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Thymidylate synthase Antibody in Western Blot (WB)

Thymidylate synthase Antibody in Flow Cytometry (Flow)

Thymidylate synthase Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15047-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,200

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (MISC)

View 3 publications 3 publications

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Thymidylate synthase Fusion Protein Ag7027 (1-313 aa encoded by BC002567)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MPVAGSELP RRPLPPAAQE RDAEPRPPHG ELQYLGQIQH ILRCGVRKDD RTGTGTLSVF GMQARYSLRD EFPLLTTKRV FWKGVLEELL WFIKGSTNAK ELSSKGVKIW DANGSRDFLD SLGFSTREEG DLGPVYGFQW RHFGAEYRDM ESDYSGQGVD QLQRVIDTIK TNPDDRRIIM CAWNPRDLPL MALPPCHALC QFYVVNSELS CQLYQRSGDM GLGVPFNIAS YALLTYMIAH ITGLKPGDFI HTLGDAHIYL NHIEPLKIQL QREPRPFPKL RILRKVEKID DFKAEDFQIE GYNPHPTIKM EMAV (1-313 aa encoded by BC002567)

Target Information

Thymidylate Synthase (TS) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Therapeutic potential of Clostridium butyricum anticancer effects in colorectal cancer.

Gut microbes

Xu H,Luo H,Zhang J,Li K,Lee MH

Wed Mar 22 00:00:00 UTC 2023

Pemetrexed induces ROS generation and cellular senescence by attenuating TS-mediated thymidylate metabolism to reverse gefitinib resistance in NSCLC.

Journal of cellular and molecular medicine

Chen Y,Zhang C,Jin S,Li J,Dai J,Zhang Z,Guo R

Sat Jul 01 00:00:00 UTC 2023

Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions.

Nature metabolism

Skinner OS,Blanco-Fernández J,Goodman RP,Kawakami A,Shen H,Kemény LV,Joesch-Cohen L,Rees MG,Roth JA,Fisher DE,Mootha VK,Jourdain AA

Mon May 01 00:00:00 UTC 2023

Comprehensive serum metabolomics and network analysis to reveal the mechanism of gypenosides in treating lung cancer and enhancing the pharmacological effects of cisplatin.

Frontiers in pharmacology

Qi YS,Xiao MY,Xie P,Xie JB,Guo M,Li FF,Piao XL

Thu Dec 22 00:00:00 UTC 2022

A Prognostic Model of Differentiated Thyroid Cancer Based on Up-Regulated Glycolysis-Related Genes.

Frontiers in endocrinology

Wu M,Ou-Yang DJ,Wei B,Chen P,Shi QM,Tan HL,Huang BQ,Liu M,Qin ZE,Li N,Hu HY,Huang P,Chang S

Tue May 10 00:00:00 UTC 2022

A Prognostic Model of Differentiated Thyroid Cancer Based on Up-Regulated Glycolysis-Related Genes.

Frontiers in endocrinology

Wu M,Ou-Yang DJ,Wei B,Chen P,Shi QM,Tan HL,Huang BQ,Liu M,Qin ZE,Li N,Hu HY,Huang P,Chang S

Tue May 10 00:00:00 UTC 2022

CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity.

Cell chemical biology

Liang YY,Bacanu S,Sreekumar L,Ramos AD,Dai L,Michaelis M,Cinatl J,Seki T,Cao Y,Coffill CR,Lane DP,Prabhu N,Nordlund P

Thu Apr 21 00:00:00 UTC 2022

The Implication of Serum Autoantibodies in Prognosis of Canine Mammary Tumors.

Animals : an open access journal from MDPI

Yuan SH,Chang SC,Chou PY,Yang Y,Liu HP

Sun Sep 18 00:00:00 UTC 2022

lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients.

Oxidative medicine and cellular longevity

Liang G,Zeng M,Gao M,Xing W,Jin X,Wang Q,Deng L,Ou H,He Z

Tue Mar 08 00:00:00 UTC 2022

Significant association of serum autoantibodies to TYMS, HAPLN1 and IGFBP5 with early stage canine malignant mammary tumours.

Veterinary and comparative oncology

Chang SC,Yuan SH,Li CY,Chang HM,Wang HC,Pan YA,Hsueh PC,Wu CC,Yang Y,Liu HP

Mon Mar 01 00:00:00 UTC 2021

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Journal of pineal research

Cui L,Zhao X,Jin Z,Wang H,Yang SF,Hu S

Wed Dec 01 00:00:00 UTC 2021

Uncovering the anti-NSCLC effects and mechanisms of gypenosides by metabolomics and network pharmacology analysis.

Journal of ethnopharmacology

Qi YS,Xie JB,Xie P,Duan Y,Ling YQ,Gu YL,Piao XL

Sun Dec 05 00:00:00 UTC 2021

A Novel Integrated Metabolism-Immunity Gene Expression Model Predicts the Prognosis of Lung Adenocarcinoma Patients.

Frontiers in pharmacology

Chen S,Duan Y,Wu Y,Yang D,An J

Tue Aug 17 00:00:00 UTC 2021

Identification and Validation of TYMS as a Potential Biomarker for Risk of Metastasis Development in Hepatocellular Carcinoma.

Frontiers in oncology

Li S,Zhao J,Lv L,Dong D

Sat Dec 04 00:00:00 UTC 2021

MicroRNA-375-3p enhances chemosensitivity to 5-fluorouracil by targeting thymidylate synthase in colorectal cancer.

Cancer science

Xu F,Ye ML,Zhang YP,Li WJ,Li MT,Wang HZ,Qiu X,Xu Y,Yin JW,Hu Q,Wei WH,Chang Y,Liu L,Zhao Q

Fri May 01 00:00:00 UTC 2020

MicroRNA-375-3p enhances chemosensitivity to 5-fluorouracil by targeting thymidylate synthase in colorectal cancer.

Cancer science

Xu F,Ye ML,Zhang YP,Li WJ,Li MT,Wang HZ,Qiu X,Xu Y,Yin JW,Hu Q,Wei WH,Chang Y,Liu L,Zhao Q

Fri May 01 00:00:00 UTC 2020

5-Fluorouracil Enhances the Antitumor Activity of the Glutaminase Inhibitor CB-839 against PIK3CA-Mutant Colorectal Cancers.

Cancer research

Zhao Y,Feng X,Chen Y,Selfridge JE,Gorityala S,Du Z,Wang JM,Hao Y,Cioffi G,Conlon RA,Barnholtz-Sloan JS,Saltzman J,Krishnamurthi SS,Vinayak S,Veigl M,Xu Y,Bajor DL,Markowitz SD,Meropol NJ,Eads JR,Wang Z

Sun Nov 01 00:00:00 UTC 2020

Nine hub genes related to the prognosis of HBV-positive hepatocellular carcinoma identified by protein interaction analysis.

Annals of translational medicine

Xie W,Wang B,Wang X,Hou D,Su H,Huang H

Wed Apr 01 00:00:00 UTC 2020

Pemetrexed inhibits Kaposi's sarcoma-associated herpesvirus replication through blocking dTMP synthesis.

Antiviral research

Chen J,Zhang H,Chen X

Sat Aug 01 00:00:00 UTC 2020

Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.

International journal of cancer

Fu F,Zhang Y,Gao Z,Zhao Y,Wen Z,Han H,Li Y,Chen H

Wed Jul 15 00:00:00 UTC 2020

MEK inhibitor enhanced the antitumor effect of oxaliplatin and 5‑fluorouracil in MEK1 Q56P‑mutant colorectal cancer cells.

Molecular medicine reports

Jing C,Li H,Du Y,Cao H,Liu S,Wang Z,Ma R,Feng J,Wu J

Fri Feb 01 00:00:00 UTC 2019

MEK inhibitor enhanced the antitumor effect of oxaliplatin and 5‑fluorouracil in MEK1 Q56P‑mutant colorectal cancer cells.

Molecular medicine reports

Jing C,Li H,Du Y,Cao H,Liu S,Wang Z,Ma R,Feng J,Wu J

Fri Feb 01 00:00:00 UTC 2019

An efficient proteome-wide strategy for discovery and characterization of cellular nucleotide-protein interactions.

PloS one

Lim YT,Prabhu N,Dai L,Go KD,Chen D,Sreekumar L,Egeblad L,Eriksson S,Chen L,Veerappan S,Teo HL,Tan CSH,Lengqvist J,Larsson A,Sobota RM,Nordlund P

Mon May 06 00:00:00 UTC 2019

An efficient proteome-wide strategy for discovery and characterization of cellular nucleotide-protein interactions.

PloS one

Lim YT,Prabhu N,Dai L,Go KD,Chen D,Sreekumar L,Egeblad L,Eriksson S,Chen L,Veerappan S,Teo HL,Tan CSH,Lengqvist J,Larsson A,Sobota RM,Nordlund P

Mon May 06 00:00:00 UTC 2019

Inhibition of polypyrimidine tract-binding protein 3 induces apoptosis and cell cycle arrest, and enhances the cytotoxicity of 5- fluorouracil in gastric cancer cells.

British journal of cancer

Liang X,Shi H,Yang L,Qiu C,Lin S,Qi Y,Li J,Zhao A,Liu J

Tue Mar 28 00:00:00 UTC 2017

Inhibition of polypyrimidine tract-binding protein 3 induces apoptosis and cell cycle arrest, and enhances the cytotoxicity of 5- fluorouracil in gastric cancer cells.

British journal of cancer

Liang X,Shi H,Yang L,Qiu C,Lin S,Qi Y,Li J,Zhao A,Liu J

Tue Mar 28 00:00:00 UTC 2017

The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.

Cell reports

Hoxhaj G,Hughes-Hallett J,Timson RC,Ilagan E,Yuan M,Asara JM,Ben-Sahra I,Manning BD

Tue Oct 31 00:00:00 UTC 2017

The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.

Cell reports

Hoxhaj G,Hughes-Hallett J,Timson RC,Ilagan E,Yuan M,Asara JM,Ben-Sahra I,Manning BD

Tue Oct 31 00:00:00 UTC 2017

CETSA screening identifies known and novel thymidylate synthase inhibitors and slow intracellular activation of 5-fluorouracil.

Nature communications

Almqvist H,Axelsson H,Jafari R,Dan C,Mateus A,Haraldsson M,Larsson A,Martinez Molina D,Artursson P,Lundbäck T,Nordlund P

Thu Mar 24 00:00:00 UTC 2016

CETSA screening identifies known and novel thymidylate synthase inhibitors and slow intracellular activation of 5-fluorouracil.

Nature communications

Almqvist H,Axelsson H,Jafari R,Dan C,Mateus A,Haraldsson M,Larsson A,Martinez Molina D,Artursson P,Lundbäck T,Nordlund P

Thu Mar 24 00:00:00 UTC 2016

Suberoylanilide hydroxamic acid enhances chemosensitivity to 5-fluorouracil in hepatocellular carcinoma via inhibition of thymidylate synthase.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Liao B,Liang H,Chen J,Liu Q,Zhang B,Chen X

Tue Dec 01 00:00:00 UTC 2015

Suberoylanilide hydroxamic acid enhances chemosensitivity to 5-fluorouracil in hepatocellular carcinoma via inhibition of thymidylate synthase.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Liao B,Liang H,Chen J,Liu Q,Zhang B,Chen X

Tue Dec 01 00:00:00 UTC 2015

Assay Guidance Manual

null

Sat Jan 01 00:00:00 UTC 1

Assay Guidance Manual

null

Sat Jan 01 00:00:00 UTC 1

Bioinformatics

Protein Aliases: MGC88736; Thymidylate synthase; TS; TSase; tysy

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Gene Aliases: HST422; OK/SW-cl.29; TMS; TS; TYMS

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UniProt ID: (Human) P04818, (Mouse) P07607, (Rat) P45352

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Entrez Gene ID: (Human) 7298, (Mouse) 22171, (Rat) 29261

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Function(s)

nucleotide binding mRNA binding thymidylate synthase activity folic acid binding drug binding protein homodimerization activity cofactor binding methyltransferase activity transferase activity

Process(es)

dTMP biosynthetic process nucleotide biosynthetic process methylation dUMP metabolic process G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle nucleobase-containing compound metabolic process dTTP biosynthetic process aging circadian rhythm cell proliferation deoxyribonucleoside monophosphate biosynthetic process response to toxic substance cell growth immortalization of host cell by virus uracil metabolic process organ regeneration response to progesterone response to vitamin A response to cytokine tetrahydrofolate interconversion response to drug response to ethanol pyrimidine nucleoside biosynthetic process response to organophosphorus developmental growth cartilage development response to glucocorticoid response to folic acid intestinal epithelial cell maturation DNA biosynthetic process pyrimidine nucleobase metabolic process response to organic cyclic compound tetrahydrofolate metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Thymidylate synthase Polyclonal Antibody

Thymidylate synthase Antibody (15047-1-AP) in ICC/IF

Product Details

15047-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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