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Thyroid Hormone Receptor beta 1 Antibody (600-401-A96) in ICC/IF
Immunofluorescence microscopy anti -THRB1 (Thyroid hormone receptor Beta 1) antibody 600-401-A96. Tissue: Mouse Dendritic cells. Primary antibody: Anti THRB1 1:100 1 hr PBS 3% BSA (left) Normal rabbit IgG isotype control (right). Secondary Ab: 488 dye conjugate 1:1000 1 hr. Mounting: Fluoromount-G (Southern Biotechnology Associates, Birmingham, AL) for examination. This image appeared originally in Mascanf... View More
Product Details
600-401-A96
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-TR ß1 antibody is affinity purified from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human THRB isoform 1 protein.
Conjugate
Form
Liquid
Concentration
1.15 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody reacts with human THRB protein. A BLAST analysis was used to suggest cross-reactivity with THRB from mouse, human and rat based on a 100% homology with the immunizing sequence. Cross-reactivity with THRB from other sources has not been determined.
Target Information
Thyroid hormone receptors (TR), also known as c-erbA-2, are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1. The corresponding gene for the thyroid hormone receptor beta-1 is NR1A2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-erbA-2; c-erbA-beta; ERBA 2; ERBA BETA; ERBA-BETA; MGC126109; MGC126110; Nuclear receptor subfamily 1 group A member 2; oncogene ERBA2; thyroid hormone nuclear receptor beta variant 1; Thyroid hormone receptor beta; thyroid hormone receptor beta 3; thyroid hormone receptor beta2delta; thyroid hormone receptor, beta (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 2); thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian); TR; TR beta; TR beta 1; TR-B1C-ERBA-2; TRbeta2Delta
Gene Aliases: C-ERBA-2; C-ERBA-BETA; c-erbAbeta; ERBA2; GRTH; NR1A2; PRTH; RATT3REC; T3R[b]; T3Rbeta; T3rec; THR1; THRB; THRB1; THRB2; TRbeta
UniProt ID: (Human) P10828, (Mouse) P37242
Entrez Gene ID: (Human) 7068, (Mouse) 21834, (Rat) 24831
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
transcription corepressor activity
thyroid hormone receptor activity
protein binding
zinc ion binding
enzyme binding
chromatin DNA binding
sequence-specific DNA binding
thyroid hormone binding
double-stranded DNA binding
protein homodimerization activity
metal ion binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
sensory perception of sound
negative regulation of female receptivity
regulation of heart contraction
female courtship behavior
positive regulation of cell proliferation
organ morphogenesis
regulation of lipid metabolic process
intracellular receptor signaling pathway
positive regulation of chondrocyte differentiation
steroid hormone mediated signaling pathway
positive regulation of ossification
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
Type I pneumocyte differentiation
regulation of cholesterol metabolic process
regulation of triglyceride metabolic process
transcription initiation from RNA polymerase II promoter
embryonic organ development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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