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Timp-3 Polyclonal Antibody

Timp-3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Timp-3 Antibody (10858-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10858-1-AP (Timp-3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

10858-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:800

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Timp-3 Fusion Protein Ag1253

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

247 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

Tissue inhibitor of metalloproteinase 3 (TIMP3) is a member of the tissue inhibitor of metalloproteinases gene family. It functions to inhibit the activity of matrix metalloproteinases (MMP) which degrade components of the extracellular matrix. TIMP3 is expressed upon mitogenic stimulation. It binds irreversibly to zinc-dependent MMPs and inactivates them by binding to their catalytic zinc cofactor. Other functions of TIMP3 include nervous system development, tissue regeneration, and visual perception. In humans, the gene encoding TIMP3 is located on chromosome 22.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Methyltransferase-like 3 suppresses phenotypic switching of vascular smooth muscle cells by activating autophagosome formation.

Cell proliferation

Fang ZM,Zhang SM,Luo H,Jiang DS,Huo B,Zhong X,Feng X,Cheng W,Chen Y,Feng G,Wu X,Zhao F,Yi X

Sat Apr 01 00:00:00 UTC 2023

Protective role of Achyranthes bidentata polysaccharides against chondrocyte extracellular matrix degeneration through lncRNA GAS5 in osteoarthritis.

Experimental and therapeutic medicine

Fu C,Qiu Z,Huang Y,Mei Y,Lin Q,Zeng J,Zhong W,Ma D

Mon Aug 01 00:00:00 UTC 2022

Investigating the mechanism of Xian-ling-lian-xia-fang for inhibiting vasculogenic mimicry in triple negative breast cancer via blocking VEGF/MMPs pathway.

Chinese medicine

Li F,Shi Y,Zhang Y,Yang X,Wang Y,Jiang K,Hua C,Wu C,Sun C,Qin Y,Liu S

Mon Apr 04 00:00:00 UTC 2022

Tau Protein Modulates Perineuronal Extracellular Matrix Expression in the TauP301L-acan Mouse Model.

Biomolecules

Schmidt S,Holzer M,Arendt T,Sonntag M,Morawski M

Sat Mar 26 00:00:00 UTC 2022

Trehalose Protects Keratinocytes against Ultraviolet B Radiation by Activating Autophagy via Regulating TIMP3 and ATG9A.

Oxidative medicine and cellular longevity

Li L,Chen H,Chen X,Chen S,Gu H

Mon Apr 25 00:00:00 UTC 2022

Prostaglandin-E2 receptor-4 stimulant rescues cardiac malfunction during myocarditis and protects the heart from adverse ventricular remodeling after myocarditis.

Scientific reports

Takakuma A,Nishii M,Valaperti A,Hiraga H,Saji R,Sakai K,Matsumura R,Miyata Y,Oba N,Nunose F,Ogawa F,Tamura K,Takeuchi I

Tue Oct 26 00:00:00 UTC 2021

Effects of aging on the histology and biochemistry of rat tendon healing.

BMC musculoskeletal disorders

Lai F,Tang H,Wang J,Lu K,Bian X,Wang Y,Shi Y,Guo Y,He G,Zhou M,Zhang X,Zhou B,Zhang J,Chen W,Tang K

Mon Nov 15 00:00:00 UTC 2021

The prognostic and diagnostic value of tissue inhibitor of metalloproteinases gene family and potential function in gastric cancer.

Journal of Cancer

Li Z,Jing Q,Wu L,Chen J,Huang M,Qin Y,Wang T

Tue Jun 08 00:00:00 UTC 2021

MicroRNA-221 promotes proliferation and migration of pulmonary arterial smooth muscle cells (PASMCs) by targeting tissue inhibitor of metalloproteinases-3 (TIMP3).

Cardiovascular diagnosis and therapy

Yan Y,Xu Y,Ni G,Wang S,Li X,Gao J,Zhang H

Sat Aug 01 00:00:00 UTC 2020

Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT-3 signalling pathway.

Cell proliferation

Wang Y,He G,Tang H,Shi Y,Kang X,Lyu J,Zhu M,Zhou M,Yang M,Mu M,Chen W,Zhou B,Zhang J,Tang K

Mon Jul 01 00:00:00 UTC 2019

miR-222-3p promotes osteosarcoma cell migration and invasion through targeting TIMP3.

OncoTargets and therapy

Guo J,Liu Q,Li Z,Guo H,Bai C,Wang F

Wed Oct 04 00:00:00 UTC 2023

Databases for technical aspects of immunohistochemistry.

Journal of toxicologic pathology

Furukawa S,Nagaike M,Ozaki K

Sun Jan 01 00:00:00 UTC 2017

miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.

Theranostics

Shi J,Bei Y,Kong X,Liu X,Lei Z,Xu T,Wang H,Xuan Q,Chen P,Xu J,Che L,Liu H,Zhong J,Sluijter JP,Li X,Rosenzweig A,Xiao J

Mon Oct 23 00:00:00 UTC 2017

AMPK deficiency in chondrocytes accelerated the progression of instability-induced and ageing-associated osteoarthritis in adult mice.

Scientific reports

Zhou S,Lu W,Chen L,Ge Q,Chen D,Xu Z,Shi D,Dai J,Li J,Ju H,Cao Y,Qin J,Chen S,Teng H,Jiang Q

Wed Feb 22 00:00:00 UTC 2017

Lack of Correlation between Aberrant p16, RAR-β2, TIMP3, ERCC1, and BRCA1 Protein Expression and Promoter Methylation in Squamous Cell Carcinoma Accompanying Candida albicans-Induced Inflammation.

PloS one

Terayama Y,Matsuura T,Ozaki K

Mon Jul 31 00:00:00 UTC 2017

Involvement of calcium-sensing receptors in hypoxia-induced vascular remodeling and pulmonary hypertension by promoting phenotypic modulation of small pulmonary arteries.

Molecular and cellular biochemistry

Peng X,Li HX,Shao HJ,Li GW,Sun J,Xi YH,Li HZ,Wang XY,Wang LN,Bai SZ,Zhang WH,Zhang L,Yang GD,Wu LY,Wang R,Xu CQ

Sat Nov 01 00:00:00 UTC 2014

Elevated MiR-222-3p promotes proliferation and invasion of endometrial carcinoma via targeting ERα.

PloS one

Liu B,Che Q,Qiu H,Bao W,Chen X,Lu W,Li B,Wan X

Wed Oct 15 00:00:00 UTC 2014

EZH2 regulates cancer cell migration through repressing TIMP-3 in non-small cell lung cancer.

Medical oncology (Northwood, London, England)

Xu C,Hou Z,Zhan P,Zhao W,Chang C,Zou J,Hu H,Zhang Y,Yao X,Yu L,Yan J

Sun Dec 01 00:00:00 UTC 2013

Timp-3 deficiency impairs cognitive function in mice.

Laboratory investigation; a journal of technical methods and pathology

Baba Y,Yasuda O,Takemura Y,Ishikawa Y,Ohishi M,Iwanami J,Mogi M,Doe N,Horiuchi M,Maeda N,Fukuo K,Rakugi H

Tue Dec 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: Metalloproteinase inhibitor 3; MIG-5 protein; Protein MIG-5; TIMP 3; TIMP-3; Tissue inhibitor of metalloproteinases 3

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Gene Aliases: HSMRK222; K222; K222TA2; SFD; Sun; Timp-3; TIMP3

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UniProt ID: (Human) P35625, (Mouse) P39876

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Entrez Gene ID: (Human) 7078, (Mouse) 21859

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Function(s)

protease binding protein binding metalloendopeptidase inhibitor activity metal ion binding enzyme inhibitor activity peptidase inhibitor activity

Process(es)

platelet degranulation central nervous system development aging visual perception response to mechanical stimulus response to hormone response to organic substance tissue regeneration response to amino acid response to estrogen negative regulation of membrane protein ectodomain proteolysis response to folic acid negative regulation of ERK1 and ERK2 cascade cellular response to organic substance positive regulation of TRAIL-activated apoptotic signaling pathway negative regulation of metalloendopeptidase activity negative regulation of peptidase activity negative regulation of endopeptidase activity response to cytokine negative regulation of catalytic activity negative regulation of proteolysis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Timp-3 Polyclonal Antibody

Timp-3 Antibody (10858-1-AP) in IHC (P)

Product Details

10858-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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