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Bioss
Titin Polyclonal Antibody
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FIGURE: 1 / 2
Titin Antibody (BS-9861R) in Flow
U2OS cells (black) were fixed with 4% PFA for 10 min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CMD1G Antibody (bs-9861R) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Product Details
BS-9861R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human Titin (Ig-like 15 domain), amino acids 2670-2703.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Monoclonal Anti-Titin can be used for study of the elastic filaments within sarcomeric structures. It is also useful as a differentiation marker in the separation of rhabdomyosarcomas from other muscle tumors. By indirect immunofluorescence the antibody displays a typical striated staining pattern on frozen sections of chicken skeletal and cardiac muscle tissues. Stains the region of the A-I junction by indirect immunofluorescence. It shows a decoration line 0.05 mm from the end of the A band in electron micro-graphs. In immunoblotting using total extracts of chicken breast muscle the antibody reacts specifically with both bands of the titin doublet, and showed no reaction with nebulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2.7.11.1; Connectin; DKFZp451N061; FLJ26020; FLJ26409; FLJ32040; FLJ34413; FLJ39564; FLJ43066; Rhabdomyosarcoma antigen MU-RMS-40.14; Titin; titin protein homolog; ttn
Gene Aliases: 1100001C23Rik; 2310036G12Rik; 2310057K23Rik; 2310074I15Rik; AF006999; AV006427; CMD1G; CMH9; CMPD4; D330041I19Rik; D830007G01Rik; EOMFC; HMERF; L56; LGMD2J; mdm; MYLK5; shru; TMD; TTN
UniProt ID: (Human) Q8WZ42
Entrez Gene ID: (Human) 7273, (Rat) 84015, (Mouse) 22138
protease binding
protein serine/threonine kinase activity
protein tyrosine kinase activity
calcium ion binding
protein binding
calmodulin binding
ATP binding
structural constituent of muscle
enzyme binding
protein kinase binding
telethonin binding
identical protein binding
actinin binding
protein self-association
actin filament binding
muscle alpha-actinin binding
structural molecule activity conferring elasticity
structural constituent of cytoskeleton
in utero embryonic development
heart morphogenesis
cardiac muscle hypertrophy
muscle contraction
mitotic chromosome condensation
adult heart development
peptidyl-tyrosine phosphorylation
ventricular system development
skeletal muscle thin filament assembly
skeletal muscle myosin thick filament assembly
detection of muscle stretch
forward locomotion
sarcomere organization
regulation of protein kinase activity
cardiac muscle fiber development
sarcomerogenesis
regulation of catalytic activity
response to calcium ion
striated muscle cell development
cardiac myofibril assembly
cardiac muscle tissue morphogenesis
cardiac muscle contraction
heart growth
platelet degranulation
striated muscle contraction
muscle filament sliding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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