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Tlk1 Antibody (600-401-469) in WB
Western blot using Rocklands Affinity Purified anti-Tlk-1 antibody shows detection of a band ~64 kDa corresponding to isoform 3 of human Tlk-1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 30 µg of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:250 for 2h at room temperature followed by washes and reaction with a 1:10,... View More
Product Details
600-401-469
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chimpanzee,
Human,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 730-755 of human Tlk1 (Tousled-like kinase) (isoform 1) protein.
Conjugate
Form
Liquid
Concentration
0.6 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human Tlk-1 protein. This antibody reacts with both phosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) of the protein. The antibody will react with all isoforms of Tlk-1. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, and dog sources based on 100% homology for the immunogen sequence. Cross reactivity with Tlk-1 protein from mouse, rat and chicken may occur as this sequence only varies by one amino acid residue (93% homology). Cross reactivity with Tlk-1 homologues from other sources has not been determined.
Target Information
TLK1 or tousled-like kinase 1 is a nuclear serine/threonine kinase that is potentially involved in the regulation of chromatin assembly. TLK1 is first described in Arabidopsis. TLK1 is a novel target of the DNA damage checkpoint that is rapidly inactivated upon exposure to ionizing radiation (IR) and the inactivation is directly mediated by the S-phase DNA damage checkpoint. The suppression of TLK1 activity after double-strand breaks in the DNA, replication blockade, or low doses of ultraviolet irradiation requires ATM, NBS and CHK1. The Tousled-like kinases, first described in Arabadopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PKU beta; PKU-beta; serine threonine protein kinase; Serine/threonine-protein kinase tousled-like 1; SNAK; SNARE protein kinase SNAK; TLK-1; TLK1B; Tousled-like kinase 1
Gene Aliases: KIAA0137; PKU-beta; TLK1
UniProt ID: (Human) Q9UKI8
Entrez Gene ID: (Human) 9874, (Dog) 100856355
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