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Invitrogen
Transferrin Receptor Monoclonal Antibody (DF1513)
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FIGURE: 1 / 4
Transferrin Receptor Antibody (MA5-16643) in ICC/IF
Immunofluorescence analysis of Transferrin Receptor was performed using 70% confluent log phase HeLa cells treated with BrefeldinA (5 µg/mL for 30 minutes). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Transferrin Receptor Monoclonal Antibody (Product # M... View More
Product Details
MA5-16643
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mustelid,
Rhesus monkey
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
DF1513
Immunogen
KG1 cell line
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2538138
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Human CD71 antibody, clone DF1513 recognizes the human CD71 cell surface antigen, a approximately 190 kDa homodimeric glycoprotein expressed by proliferating cells.
Target Information
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD antigen CD71; CD71; p90; T9; TR; transferrin receptor 1; Transferrin receptor protein 1
Gene Aliases: CD71; IMD46; p90; T9; TFR; TFR1; TFRC; TR; TRFR
UniProt ID: (Human) P02786
Entrez Gene ID: (Human) 7037, (Rhesus monkey) 711568
regulation of cell growth
response to hypoxia
cellular iron ion homeostasis
acute-phase response
aging
response to nutrient
response to iron ion
response to manganese ion
osteoclast differentiation
positive regulation of B cell proliferation
receptor internalization
response to retinoic acid
transferrin transport
cellular response to drug
positive regulation of T cell proliferation
regulation of cell proliferation
positive regulation of bone resorption
positive regulation of isotype switching
response to copper ion
viral entry into host cell
iron ion import
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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