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Invitrogen
Troponin I Recombinant Rabbit Monoclonal Antibody (1H11L19)
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Troponin I Antibody (701585) in ICC/IF
Immunofluorescent analysis of Troponin I was done on Primary Human Cardiomyocytes. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Troponin I Recombinant Rabbit Monoclonal Antibody (Product # 701585) at a dilution of 1:400 in 1% BSA and incubated for 3 hour... View More
Product Details
701585
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
1H11L19
Immunogen
human Troponin I
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532494
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); DKFZp451O223; Tpn1; troponin 1, type 3; troponin I type 1 (skeletal, slow); troponin I type 3 (cardiac); Troponin I, cardiac muscle; troponin I, skeletal, slow 1; Troponin I, slow isoform; Troponin I, slow skeletal muscle; Troponin I, slow-twitch isoform
Gene Aliases: 2700018B22Rik; AI747285; CMD1FF; CMD2A; CMH7; Ctni; RCM1; SSTNI; Tn1; TnI; TNN1; TNNC1; TNNI1; TNNI3
UniProt ID: (Human) P19237, (Human) P19429, (Mouse) Q9WUZ5, (Rat) P13413, (Rat) P23693, (Mouse) P48787
Entrez Gene ID: (Human) 7135, (Human) 7137, (Mouse) 21952, (Rat) 29388, (Rat) 29248, (Mouse) 21954
skeletal muscle contraction
regulation of striated muscle contraction
transition between fast and slow fiber
muscle filament sliding
ventricular cardiac muscle tissue morphogenesis
cardiac muscle contraction
regulation of muscle contraction
vasculogenesis
regulation of systemic arterial blood pressure by ischemic conditions
cellular calcium ion homeostasis
regulation of smooth muscle contraction
striated muscle contraction
heart development
negative regulation of ATPase activity
heart contraction
regulation of cardiac conduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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