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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Tyrosinase Antibodies

          Invitrogen

          Tyrosinase Monoclonal Antibody (T311)

          Advanced Verification
          2 Published Figures
          25 References
          View all (43) Tyrosinase antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite Tyrosinase Monoclonal Antibody (T311)

          • Antibody Testing Data (3)
          • Published Figures (2)
          • Advanced Verification (1)
          Tyrosinase Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Tyrosinase Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          Tyrosinase Antibody (MA5-14177) in ICC/IF

          Immunofluorescence analysis of Tyrosinase was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Tyrosinase Monoclonal Antibody (T311) (Product # MA5-14177) at 1:250 dilution in 0.1% BSA, incubated at 4 degre... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Tyrosinase Antibody in Immunocytochemistry (ICC/IF)
          Tyrosinase Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Tyrosinase Antibody in Western Blot (WB)
          Tyrosinase Antibody in Western Blot (WB)
          Tyrosinase Antibody in Western Blot (WB)
          Tyrosinase Antibody

          Product Details

          MA5-14177

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:25-1:100
          View 6 publications 6 publications

          Immunohistochemistry (IHC)

          -
          View 12 publications 12 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:100
          -

          Immunocytochemistry (ICC/IF)

          1:250
          View 3 publications 3 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          T311

          Immunogen

          Recombinant tyrosinase protein

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Protein A/G

          Storage buffer

          PBS, pH 7.4, with 0.05% BSA

          Contains

          0.05% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10980000

          Product Specific Information

          MA5-14177 targets Tyrosinase in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples.

          The MA5-14177 immunogen is recombinant tyrosinase protein.

          Target Information

          The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Albino locus protein; LB24-AB; Monophenol monooxygenase; oculocutaneous albinism IA; SK29-AB; Tumor rejection antigen AB; Tyrosinase

          View more View less

          Gene Aliases: albino; ATN; c; CMM8; OCA1; OCA1A; OCAIA; SHEP3; skc35; TYR

          View more View less

          UniProt ID: (Human) P14679, (Mouse) P11344

          View more View less

          Entrez Gene ID: (Human) 7299, (Mouse) 22173

          View more View less

          Function(s)
          monooxygenase activity monophenol monooxygenase activity copper ion binding protein binding protein homodimerization activity protein heterodimerization activity oxidoreductase activity metal ion binding
          Process(es)
          metabolic process cell proliferation melanin biosynthetic process pigmentation thymus development oxidation-reduction process melanin biosynthetic process from tyrosine eye pigment biosynthetic process visual perception response to UV response to vitamin D response to cAMP
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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