Invitrogen

Tyrosine Hydroxylase Polyclonal Antibody

Tyrosine Hydroxylase Antibody in Immunocytochemistry (ICC/IF)

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Tyrosine Hydroxylase Antibody (OPA1-04050) in ICC/IF

Immunofluorescence analysis of Anti-Tyrosine Hydroxylase Polyclonal Antibody (Product # OPA1-04050) was performed using PC-12 cells treated with 1 µg/mL dexamethasone for 6 days. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Tyrosine Hydroxylase Polycl... View More

Tyrosine Hydroxylase Antibody in Western Blot (WB)

Tyrosine Hydroxylase Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Tyrosine Hydroxylase Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))

Tyrosine Hydroxylase Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Product Details

OPA1-04050

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

1:1,000

View 9 publications 9 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

View 2 publications 2 publications

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:1,000

Product Specifications

Species Reactivity

Human, Many, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 0.1mg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_325653

Product Specific Information

In Western blot, this antibody detects a single ~60 kDa protein representing tyrosine hydroxylase from rat brain lysates of PC-12 cells stimulated by okadaic acid. Immunohistochemical staining of TH in human brain with OPA1-04050 results in intense labeling of the dopaminergic neurons in the substantia nigra.

Store at -20ºC short term, 80ºC long term.

Target Information

Tyrosine hydroxylase (TH) is an enzyme involved in the synthesis of catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine. In all species, catecholamine synthesis is regulated by the interaction of TH with a cofactor, tetrahydrobiopterin (BH4). BH4 binds to the TH catalytic domain, resulting in enzymatic activity. Unlike TH in non-primate species, four human TH mRNA splice variants (hTH1-hTH4) have been isolated. These variants are identical in their catalytic domain, but differ in their N-terminal, regulatory domains. TH is also responsible for the conversion of L-tyrosine to L-dopa. TH plays a key role in the physiology of adrenergic neurons. The role of TH in the synthesis of catecholamine neurotransmitters suggests a correlation between the enzyme and a number of neuropathogenic diseases including: Parkinson's disease, schizophrenia, Segawa syndrome, and dystonia, as well as a variety of cardiovascular diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Could the Urease of the Gut Bacterium Proteus mirabilis Play a Role in the Altered Gut-Brain Talk Associated with Parkinson's Disease?

Microorganisms

Grahl MVC,Andrade BDS,Perin APA,Neves GA,Duarte LS,Uberti AF,Hohl KS,Follmer C,Carlini CR

Published figure using Tyrosine Hydroxylase polyclonal antibody (Product # OPA1-04050) in Western Blot

Wed Aug 09 00:00:00 UTC 2023

Inhibition of carnitine palmitoyl-transferase 1 is a potential target in a mouse model of Parkinson's disease.

NPJ Parkinson's disease

Trabjerg MS,Andersen DC,Huntjens P,Mørk K,Warming N,Kullab UB,Skjønnemand MN,Oklinski MK,Oklinski KE,Bolther L,Kroese LJ,Pritchard CEJ,Huijbers IJ,Corthals A,Søndergaard MT,Kjeldal HB,Pedersen CFM,Nieland JDV

OPA1-04050 was used in Western Blotting to confirm that rotenone induces gut dysbiosis in C57Bl/6J and, for the first time, in Park2 knockout mice and show that this dysbiosis is alleviated by the downregulation of the lipid metabolism via CPT1.

Sat Jan 21 00:00:00 UTC 2023

Axo-glial interactions between midbrain dopamine neurons and oligodendrocyte lineage cells in the anterior corpus callosum.

Brain structure & function

Caldwell M,Ayo-Jibunoh V,Mendoza JC,Brimblecombe KR,Reynolds LM,Zhu Jiang XY,Alarcon C,Fiore E,N Tomaio J,Phillips GR,Mingote S,Flores C,Casaccia P,Liu J,Cragg SJ,McCloskey DP,Yetnikoff L

OPA1-04050 was used in Immunohistochemistry to provide evidence implicating midbrain dopamine neurons in the innervation of oligodendrocyte lineage cells in the anterior corpus callosum and nearby white matter tracts of male and female adult mice.

Wed Nov 01 00:00:00 UTC 2023

Identifying dominant-negative actions of a dopamine transporter variant in patients with parkinsonism and neuropsychiatric disease.

JCI insight

Herborg F,Jensen KL,Tolstoy S,Arends NV,Posselt LP,Shekar A,Aguilar JI,Lund VK,Erreger K,Rickhag M,Lycas MD,Lonsdale MN,Rahbek-Clemmensen T,Sørensen AT,Newman AH,Løkkegaard A,Kjærulff O,Werge T,Møller LB,Matthies HJ,Galli A,Hjermind LE,Gether U

Published figure using Tyrosine Hydroxylase polyclonal antibody (Product # OPA1-04050) in Western Blot

Wed Sep 22 00:00:00 UTC 2021

Locus coeruleus spiking differently correlates with S1 cortex activity and pupil diameter in a tactile detection task.

eLife

Yang H,Bari BA,Cohen JY,O'Connor DH

OPA1-04050 was used in Immunohistochemistry to stud the relationships between activity in the locus coeruleus (LC), activity in the primary somatosensory cortex (S1), and pupil diameter in mice performing a tactile detection task.

Mon Mar 15 00:00:00 UTC 2021

Locus coeruleus spiking differently correlates with S1 cortex activity and pupil diameter in a tactile detection task.

eLife

Yang H,Bari BA,Cohen JY,O'Connor DH

Mon Mar 15 00:00:00 UTC 2021

Unbiased identification of novel transcription factors in striatal compartmentation and striosome maturation.

eLife

Cirnaru MD,Song S,Tshilenge KT,Corwin C,Mleczko J,Galicia Aguirre C,Benlhabib H,Bendl J,Apontes P,Fullard J,Creus-Muncunill J,Reyahi A,Nik AM,Carlsson P,Roussos P,Mooney SD,Ellerby LM,Ehrlich ME

OPA1-04050 was used in Immunohistochemistry (PFA fixed), Immunohistochemistry to dissect and manipulate the transcription factor networks regulating medium spiny neurone compartmentation and differentiation,.

Tue Oct 05 00:00:00 UTC 2021

Unbiased identification of novel transcription factors in striatal compartmentation and striosome maturation.

eLife

Cirnaru MD,Song S,Tshilenge KT,Corwin C,Mleczko J,Galicia Aguirre C,Benlhabib H,Bendl J,Apontes P,Fullard J,Creus-Muncunill J,Reyahi A,Nik AM,Carlsson P,Roussos P,Mooney SD,Ellerby LM,Ehrlich ME

Tue Oct 05 00:00:00 UTC 2021

Unbiased identification of novel transcription factors in striatal compartmentation and striosome maturation.

eLife

Cirnaru MD,Song S,Tshilenge KT,Corwin C,Mleczko J,Galicia Aguirre C,Benlhabib H,Bendl J,Apontes P,Fullard J,Creus-Muncunill J,Reyahi A,Nik AM,Carlsson P,Roussos P,Mooney SD,Ellerby LM,Ehrlich ME

Tue Oct 05 00:00:00 UTC 2021

Failure of Diphtheria Toxin Model to Induce Parkinson-Like Behavior in Mice.

International journal of molecular sciences

Valek L,Tegeder I

Published figure using Tyrosine Hydroxylase polyclonal antibody (Product # OPA1-04050) in Immunocytochemistry

Tue Aug 31 00:00:00 UTC 2021

RhoA in tyrosine hydroxylase neurones regulates food intake and body weight via altered sensitivity to peripheral hormones.

Journal of neuroendocrinology

Skov LJ,Ratner C,Hansen NW,Thompson JJ,Egerod KL,Burm H,Dalbøge LS,Hedegaard MA,Brakebusch C,Pers TH,Perrier JF,Holst B

OPA1-04050 was used in Western Blotting to indicate that TH neurones in ARC are important for the regulation of body weight homeostasis and that RhoA is both a central effector in these neurones and important for the development of obesity-induced ghrelin resistance.

Mon Jul 01 00:00:00 UTC 2019

Nicotine aversion is mediated by GABAergic interpeduncular nucleus inputs to laterodorsal tegmentum.

Nature communications

Wolfman SL,Gill DF,Bogdanic F,Long K,Al-Hasani R,McCall JG,Bruchas MR,McGehee DS

OPA1-04050 was used in Immunohistochemistry to show that IPN projections to the laterodorsal tegmentum (LDTg) are GABAergic using optogenetics in tissue slices from mouse brain.

Fri Jul 13 00:00:00 UTC 2018

Systemically administered neuregulin-1β1 rescues nigral dopaminergic neurons via the ErbB4 receptor tyrosine kinase in MPTP mouse models of Parkinson's disease.

Journal of neurochemistry

Depboylu C,Rösler TW,de Andrade A,Oertel WH,Höglinger GU

OPA1-04050 was used in immunohistochemistry - free floating to assess the effects of peripherally administered Nrg1beta1 in a toxin-based mouse model of Parkinson's disease

Fri May 01 00:00:00 UTC 2015

Transcriptional and structural plasticity of tyrosine hydroxylase expressing neurons in both striatum and nucleus accumbens following dopaminergic denervation.

Journal of chemical neuroanatomy

Depboylu C,Klietz M,Maurer L,Oertel WH,Kobayashi K,Weihe E,Höglinger GU,Schäfer MK

OPA1-04050 was used in immunohistochemistry to use transgenic mice to investigate the regulation of tyrosine hydroxylase positive cells following prolonged dopaminergic denervation

Sat Nov 01 00:00:00 UTC 2014

Brain-resident microglia predominate over infiltrating myeloid cells in activation, phagocytosis and interaction with T-lymphocytes in the MPTP mouse model of Parkinson disease.

Experimental neurology

Depboylu C,Stricker S,Ghobril JP,Oertel WH,Priller J,Höglinger GU

OPA1-04050 was used in immunohistochemistry to study the roles of brain-resident microglial cells and infiltrating myeloid cells in a murine model of Parkinson's disease

Sat Dec 01 00:00:00 UTC 2012

The L-type channel antagonist isradipine is neuroprotective in a mouse model of Parkinson's disease.

Neurobiology of disease

Ilijic E,Guzman JN,Surmeier DJ

OPA1-04050 was used in immunohistochemistry to investigate the therapeutic effect of L-type channel antagonist isradipine in mouse Parkinson disease model

Mon Aug 01 00:00:00 UTC 2011

Changes in serotoninergic and noradrenergic descending pain pathways during painful diabetic neuropathy: the preventive action of IGF1.

Neurobiology of disease

Morgado C,Silva L,Pereira-Terra P,Tavares I

OPA1-04050 was used in immunohistochemistry to investigate the effect of IGF1 on painful diabetic neuropathy

Fri Jul 01 00:00:00 UTC 2011

Cell type specific sequestration of choline acetyltransferase and tyrosine hydroxylase within Lewy bodies.

Acta neuropathologica

Dugger BN,Dickson DW

OPA1-04050 was used in immunohistochemistry to investigate the distribution of choline acetyltransferase and tyrosine hydroxylase in cholinergic and catecholaminergic neurons

Mon Nov 01 00:00:00 UTC 2010

Incidental Lewy body disease and preclinical Parkinson disease.

Archives of neurology

DelleDonne A,Klos KJ,Fujishiro H,Ahmed Z,Parisi JE,Josephs KA,Frigerio R,Burnett M,Wszolek ZK,Uitti RJ,Ahlskog JE,Dickson DW

OPA1-04050 was used in immunohistochemistry to determine whether incidental Lewy body disease (iLBD) is associated with preclinical Parkinson disease

Fri Aug 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: dystonia 14; EC 1.14.16.2; HGNC:11782; TH; TH isoform 3; TH isoform a; TH-4; TY3H; TYH antibody; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase

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Gene Aliases: DYT14; DYT5b; TH; The; TYH

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UniProt ID: (Human) P07101, (Mouse) P24529, (Rat) P04177

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Entrez Gene ID: (Human) 7054, (Mouse) 21823, (Rat) 25085

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Function(s)

tyrosine 3-monooxygenase activity protein binding ferrous iron binding ferric iron binding amino acid binding oxygen binding enzyme binding protein domain specific binding tetrahydrobiopterin binding dopamine binding monooxygenase activity iron ion binding oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen metal ion binding

Process(es)

response to hypoxia synaptic transmission, dopaminergic dopamine biosynthetic process from tyrosine heart development visual perception learning memory mating behavior locomotory behavior regulation of heart contraction aromatic amino acid family metabolic process organ morphogenesis aminergic neurotransmitter loading into synaptic vesicle neurotransmitter biosynthetic process dopamine biosynthetic process epinephrine biosynthetic process norepinephrine biosynthetic process catecholamine biosynthetic process eye photoreceptor cell development eating behavior response to ethanol embryonic camera-type eye morphogenesis oxidation-reduction process response to amphetamine heart morphogenesis fatty acid metabolic process sphingolipid metabolic process sensory perception of sound response to water deprivation response to light stimulus response to herbicide response to salt stress anatomical structure morphogenesis response to zinc ion multicellular organism aging response to activity glycoside metabolic process phthalate metabolic process cerebral cortex development response to nutrient levels response to estradiol response to lipopolysaccharide isoquinoline alkaloid metabolic process social behavior cellular response to drug response to isolation stress response to immobilization stress terpene metabolic process circadian sleep/wake cycle response to peptide hormone pigmentation response to ether response to pyrethroid response to corticosterone response to electrical stimulus phytoalexin metabolic process cellular response to manganese ion cellular response to nicotine cellular response to glucose stimulus cellular response to growth factor stimulus response to metal ion response to organic cyclic compound response to insecticide response to nicotine response to drug response to steroid hormone cognition protein homotetramerization response to growth factor cellular response to alkaloid

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Tyrosine Hydroxylase Polyclonal Antibody

Tyrosine Hydroxylase Antibody (OPA1-04050) in ICC/IF

Product Details

OPA1-04050

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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